| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Neurofilament light polypeptide;NF-L;68 kDa neurofilament protein;Neurofilament triplet L protein;Nefl;Nf68, Nfl; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | Pig spinal cord. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-NF68 Nefl Antibody (Monoclonal, NR4) is an antibody targeting NEFL. Common applications include WB, IHC, IHC-F. Key specifications include host: Mouse; clonality: Monoclonal; clone: Clone: NR4; isotype: Mouse IgG1; reactivity: Human,Mouse,Pig,Rat; observed MW: 72 kDa; calculated MW: 61335 MW.
Boster Bio Anti-NF68 Nefl Antibody (Monoclonal, NR4) catalog # MA1070. Tested in IHC, IHC-F, WB applications. This antibody reacts with Human, Mouse, Pig, Rat.
Key elements and design rationale
- Target: NEFL — Neurofilament light polypeptide
- Antibody format: Host: Mouse; Clonality: Monoclonal; Clone: Clone: NR4; Isotype: Mouse IgG1
- Species reactivity: Human,Mouse,Pig,Rat
- Molecular weight guidance: Observed: 72 kDa; Calculated: 61335 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
Scientific background (datasheet): Neurofilaments are composed of 3 neuron-specific proteins with apparent molecular masses of 68 kD (NFL), 125 kD (NFM) and 200 kD (NFH) on SDS-gel electrophoresis. And they have a role in the maturation of regenerating myelinated axons. Neurofilament 68 (NF68), also called Neurofilament Protein, Light Chain (NFL). It is one of the most abundant cytoskeletal components of the neuron. Mutations in this gene were reported as a cause for autosomal dominant Charcot-Marie-Tooth type 2E (CMT2E) linked to chromosome 8p21. NFL was identified repeatedly in both screenings and found to interact with Myotubularin-related 2 gene, MTMR2 in both Schwann cells and neurons.
Cellular localization (datasheet): Cytoplasm, myofibril, sarcomere. Cytoplasm, cytoskeleton.
Tissue details (datasheet): Muscle specific. Located in the thin filament of striated muscle.
Sequence similarities (datasheet): Belongs to the intermediate filament family.
Research relevance and current trends
- Commonly studied in contexts related to Cell Type Marker,Cytoskeleton,Cytoskeleton/ECM,Developmental Biology,Intermediate Filaments,Lineage Markers,Lineage Specification,Neurodegenerative Disease,Neurology Process,Neuron Marker,Neuroscience,Signal Transduction,Stem Cells.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
