| Field | Specification |
|---|---|
| Target | |
| Alternative names | Neurofilament light polypeptide;NF-L;68 kDa neurofilament protein;Neurofilament triplet L protein;Nefl;Nf68, Nfl; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Pig spinal cord. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone NR4 is a mouse monoclonal antibody that recognizes neurofilament light polypeptide (gene Nefl) in human, mouse, pig and rat samples and is validated for Western blot and IHC.
Neurofilament light chain (NF-L, NEFL) is one of the three subunits of neurofilaments, the intermediate filaments of neurons, together with NF-M and NF-H. It forms the filament backbone that supports axon caliber and structure, and neurofilament light chain released from damaged neurons is widely measured as a marker of axonal injury.
| Target | Neurofilament light polypeptide (gene Nefl; UniProt P19527, rat) |
|---|---|
| Clone | NR4 |
| Host / Clonality / Isotype | Mouse / Monoclonal / Mouse IgG1 |
| Reactivity | Human, Mouse, Pig, Rat |
| Purification | Ascites |
| Form | Lyophilized |
| Formulation | 1.2% sodium acetate; 0.01 mg NaN3; plus stabilizers |
| Reconstitution | 100 µg/mL after adding 1 mL PBS |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 2–4 µg/mL (Human, Mouse, Pig, Rat) |
|---|---|
| Western blot | 1–2 µg/mL (Human, Mouse, Pig, Rat) |
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Pig spinal cord.
Reactivity Notes
The supplier lists reactivity with human, mouse, pig and rat. UniProt places neurofilament light polypeptide in the cell projection and cytoplasm. Tissue expression noted by UniProt (rat): Expressed in the dorsal root ganglion neurons (at protein level).
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
