| Field | Specification |
|---|---|
| Target | |
| Alternative names | Nuclear factor of activated T-cells, cytoplasmic 1; NF-ATc1; NFATc1; NFAT transcription complex cytosolic component; NF-ATc; NFATc; NFATC1; NFAT2, NFATC; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human NFAT2 recombinant protein (Position: Q589-K652). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes nuclear factor of activated T-cells, cytoplasmic 1 (gene NFATC1) in human samples and is validated for ELISA, IHC and Western blot. The predicted molecular weight is 101.2 kDa, and the supplier reports an observed band at about 101 kDa.
NFATc1 (NFAT2) is a calcium/calcineurin-regulated transcription factor that moves into the nucleus when dephosphorylated. It drives cytokine genes such as IL-2 in activated T cells, controls heart valve development, and serves as the master regulator of osteoclast differentiation downstream of RANKL.
| Target | Nuclear factor of activated T-cells, cytoplasmic 1 (gene NFATC1; UniProt O95644, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 101.2 kDa |
| Observed MW | 101 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human Jurkat cells, human Daudi cells, human Ramos cells, human Raji cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 8% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human NFAT2 fragment (Gln589–Lys652), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places nuclear factor of activated T-cells, cytoplasmic 1 in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Expressed in thymus, peripheral leukocytes as T-cells and spleen. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Ye Jin et al. (2023) Fluoride-induced osteoporosis via interfering with the RANKL/RANK/OPG pathway in ovariectomized rats: Oophorectomy shifted skeletal fluorosis from osteosclerosis to osteoporosis. Environmental Pollution. 10.1016/j.envpol.2023.122407
