| Field | Specification |
|---|---|
| Target | |
| Alternative names | Nuclear factor of activated T-cells, cytoplasmic 1;NF-ATc1;NFATc1;NFAT transcription complex cytosolic component;NF-ATc;NFATc;NFATC1;NFAT2, NFATC; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human NFAT2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ABDA-14 is a rabbit monoclonal antibody that recognizes nuclear factor of activated T-cells, cytoplasmic 1 (gene NFATC1) in human samples and is validated for Western blot, ICC/IF and flow cytometry. The predicted molecular weight is 101.2 kDa, and the supplier reports an observed band at about 101 kDa.
NFATc1 (NFAT2) is a calcium/calcineurin-regulated transcription factor that moves into the nucleus when dephosphorylated. It drives cytokine genes such as IL-2 in activated T cells, controls heart valve development, and serves as the master regulator of osteoclast differentiation downstream of RANKL.
| Target | Nuclear factor of activated T-cells, cytoplasmic 1 (gene NFATC1; UniProt O95644, human) |
|---|---|
| Clone | ABDA-14 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 101.2 kDa |
| Observed MW | 101 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): Ramos cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human NFAT2.
Reactivity Notes
The supplier lists reactivity with human. UniProt places nuclear factor of activated T-cells, cytoplasmic 1 in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Expressed in thymus, peripheral leukocytes as T-cells and spleen. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yang Duan et al. (2019) PC-3-Derived Exosomes Inhibit Osteoclast Differentiation by Downregulating miR-214 and Blocking NF-B Signaling Pathway. Biomed Research International. 10.1155/2019/8650846
