| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Vitamin D-binding protein; DBP; VDB; Gc-globulin; Group-specific component; Gc |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human NG2/CSPG4 recombinant protein (Position: N962-D1194). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-NG2/CSPG4 Antibody Picoband® is an antibody for CSPG4 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: CSPG4 (group specific component); UniProt: Q6UVK1
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 310 kDa
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-NG2/CSPG4 Antibody Picoband® catalog # A03394-3.
Biological background
Biological context: Involved in vitamin D transport and storage, scavenging of extracellular G-actin, enhancement of the chemotactic activity of C5 alpha for neutrophils in inflammation and macrophage activation.
Expression and localization notes: cellular localization: Secreted..
Common research applications
- Western blotting (WB): Compare CSPG4 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of CSPG4 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify CSPG4-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: Chondroitin sulfate proteoglycan 4, also known as melanoma-associated chondroitin sulfate proteoglycan (MCSP) or neuron-glial antigen 2 (NG2), is a chondroitin sulfate proteoglycan that in humans is encoded by the CSPG4 gene. CSPG4 plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. It represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Secreted.
- Research category: Actin Binding Proteins,Actin, etc.,Cancer,Cytoskeleton,Cytoskeleton/ECM,Microfilaments,Signal Transduction,Tumor Antigens,Tumor Biomarkers,Tumor Immunology,Tumor-Associated Antigens
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
