| Field | Specification |
|---|---|
| Target | |
| Alternative names | Chondroitin sulfate proteoglycan 4;Chondroitin sulfate proteoglycan NG2;Melanoma chondroitin sulfate proteoglycan;Melanoma-associated chondroitin sulfate proteoglycan;CSPG4;MCSP; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human NG2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AOFG-3 is a rabbit monoclonal antibody that recognizes chondroitin sulfate proteoglycan 4 (gene CSPG4) in human samples and is validated for Western blot, IHC and flow cytometry. The predicted molecular weight is 250.5 kDa, and the supplier reports an observed band at about 251 kDa.
CSPG4 (also called NG2 or MCSP) is a transmembrane chondroitin sulfate proteoglycan found on pericytes, oligodendrocyte precursor cells and melanoma cells. It binds collagen VI and growth factors, links to signaling and scaffold proteins, and promotes cell spreading, migration and angiogenesis. It is a common marker of pericytes and NG2 glia and is studied in melanoma.
| Target | Chondroitin sulfate proteoglycan 4 (gene CSPG4; UniProt Q6UVK1, human) |
|---|---|
| Clone | AOFG-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 250.5 kDa |
| Observed MW | 251 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): A375 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human NG2.
Reactivity Notes
The supplier lists reactivity with human. UniProt places chondroitin sulfate proteoglycan 4 in the cell membrane, apical cell membrane and cell projection. Tissue expression noted by UniProt (human): Detected in fibroblasts (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
