| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Chondroitin sulfate proteoglycan 4;Chondroitin sulfate proteoglycan NG2;Melanoma chondroitin sulfate proteoglycan;Melanoma-associated chondroitin sulfate proteoglycan;CSPG4;MCSP; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthesized peptide derived from human NG2 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-NG2 CSPG4 Rabbit Monoclonal Antibody is an antibody targeting CSPG4. Common applications include WB, IHC, Flow Cytometry. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: AOFG-3; isotype: Rabbit IgG; reactivity: Human; observed MW: 50 kDa; calculated MW: 250537 MW.
Boster Bio Anti-NG2 CSPG4 Rabbit Monoclonal Antibody catalog # M03394. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human.
Key elements and design rationale
- Target: CSPG4 — Chondroitin sulfate proteoglycan 4
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: AOFG-3; Isotype: Rabbit IgG
- Species reactivity: Human
- Molecular weight guidance: Observed: 50 kDa; Calculated: 250537 MW
Biological background
Protein function (datasheet): Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2 (VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N- terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin- mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades. .
Cellular localization (datasheet): Cell membrane ; Single-pass type I membrane protein ; Extracellular side . Apical cell membrane ; Single-pass type I membrane protein ; Extracellular side . Cell projection, lamellipodium membrane ; Single-pass type I membrane protein ; Extracellular side . Cell surface . Localized at the apical plasma membrane it relocalizes to the lamellipodia of astrocytoma upon phosphorylation by PRKCA. Localizes to the retraction fibers. Localizes to the plasma membrane of oligodendrocytes (By similarity). .
Tissue details (datasheet): Detected only in malignant melanoma cells. .
Research relevance and current trends
- Commonly studied in contexts related to Cell Biology.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
- Flow cytometry: Quantify target-positive populations in single-cell suspensions; pair with viability and isotype/FMO controls conceptually.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
