| Field | Specification |
|---|---|
| Target | |
| Alternative names | Homeobox protein Nkx-3.1; Homeobox protein NK-3 homolog A; NKX3-1; NKX3.1; NKX3A |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Nkx3.1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 19N21 is a rabbit monoclonal antibody that recognizes homeobox protein Nkx-3.1 (gene NKX3-1) in human samples and is validated for Western blot and IHC. The predicted molecular weight is 26.4 kDa, and the supplier reports an observed band at about 35 kDa.
NKX3-1 is a homeobox transcription factor that is largely restricted to prostate epithelium. It controls epithelial growth and duct formation in the developing prostate and acts as a tumor suppressor whose loss is a frequent early event in prostate cancer. It is widely used as a marker of prostate lineage.
| Target | Homeobox protein Nkx-3.1 (gene NKX3-1; UniProt Q99801, human) |
|---|---|
| Clone | 19N21 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 26.4 kDa |
| Observed MW | 35 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): human Jurkat cells, human SIHA cells, human U2OS cells, human HepG2 cells, human A549 cells, human HEL cells, human RT4 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1: 500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human prostate cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human nkx3.1.
Reactivity Notes
The supplier lists reactivity with human. UniProt places homeobox protein Nkx-3.1 in the nucleus. Tissue expression noted by UniProt (human): Highly expressed in the prostate and, at a lower level, in the testis. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
