| Field | Specification |
|---|---|
| Target | |
| Alternative names | Serine/threonine-protein kinase NLK; Nemo-like kinase; Protein LAK1; NLK; LAK1 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human NLK |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 25N97 is a rabbit monoclonal antibody that recognizes serine/threonine-protein kinase NLK in human samples and is validated for Western blot and flow cytometry. The predicted molecular weight is 58.3 kDa, and the supplier reports an observed band at about 58 kDa.
Nemo-like kinase (NLK) is a proline-directed serine/threonine kinase related to MAP kinases. Downstream of WNT5A and TAK1 it acts in non-canonical Wnt signaling, while it suppresses canonical Wnt/β-catenin output by phosphorylating TCF/LEF factors. It also regulates other transcription factors, so it is studied in cell fate decisions, development and cancer.
| Target | Serine/threonine-protein kinase NLK (gene NLK; UniProt Q9UBE8, human) |
|---|---|
| Clone | 25N97 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 58.3 kDa |
| Observed MW | 58 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): human HeLa cells, human Caco-2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human NLK.
Reactivity Notes
The supplier lists reactivity with human. UniProt places serine/threonine-protein kinase NLK in the nucleus and cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
