| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | NLR family, pyrin domain containing 4G ;Nlrp4g ; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of mouse NLRP4G. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-NLRP4G Antibody Picoband® is an antibody targeting Nlrp4g. Common applications include WB, IHC, Flow Cytometry, ELISA. Key specifications include host: Rabbit; clonality: Polyclonal; isotype: Rabbit IgG; reactivity: Mouse; observed MW: 55 kDa; calculated MW: 85411 MW.
Boster Bio Anti-NLRP4G Antibody catalog # PA1687. Tested in IHC, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Target: Nlrp4g — Nlrp4g NLR family, pyrin domain containing 4G
- Antibody format: Host: Rabbit; Clonality: Polyclonal; Isotype: Rabbit IgG
- Species reactivity: Mouse
- Molecular weight guidance: Observed: 55 kDa; Calculated: 85411 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): Meiosis-inducer required for the transition into meiosis for both female and male germ cells. In female germ cells, required for premeiotic DNA replication and subsequent events in meiotic prophase (By similarity). .
Scientific background (datasheet): Stra8, stimulated by retinoic acid 8, is expressed in embryonic ovaries just before meiotic initiation, whereas in testis it is expressed after birth. The STRA8 gene contains 9 exons. And this gene is mapped to chromosome 7q31. Retinoic acid (RA) signaling was required for Stra8 expression, and therefore initiation of meiosis, in embryonic mouse ovaries. RA was sufficient to induce Stra8 expression in embryonic testis. Cyp26-mediated RA metabolism was responsible for delaying Stra8 expression in embryonic testis. Since RA signaling regulated Stra8 expression in both embryonic ovaries and adult testis, it was said this portion of the meiotic initiation pathway may be identical in both sexes.
Cellular localization (datasheet): Cytoplasm .
Tissue details (datasheet): Expressed specifically in testis. .
Sequence similarities (datasheet): Contains DAPIN domain.
Research relevance and current trends
- Commonly studied in contexts related to Cell Biology.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- ELISA: Measure target abundance in compatible matrices using a standard-curve readout; ensure dilution linearity and appropriate controls.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
- Flow cytometry: Quantify target-positive populations in single-cell suspensions; pair with viability and isotype/FMO controls conceptually.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a polyclonal antibody, this reagent may recognize multiple epitopes on the target, which can improve detection robustness but may require careful specificity controls.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
