| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Glutamate receptor ionotropic, NMDA 2A;GluN2A;Glutamate [NMDA] receptor subunit epsilon-1;N-methyl D-aspartate receptor subtype 2A;NMDAR2A;NR2A;hNR2A;GRIN2A;NMDAR2A; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human NMDAR2A recombinant protein (Position: D958-R1300). Human NMDAR2A shares 89% and 90% amino acid (aa) sequence identity with mouse and rat NMDAR2A, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of GRIN2A (Glutamate receptor ionotropic, NMDA 2A) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-NMDAR2A/GRIN2A Antibody Picoband® catalog # PB9335. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E.coli-derived human NMDAR2A recombinant protein (Position: D958-R1300). Human NMDAR2A shares 89% and 90% amino acid (aa) sequence identity with mouse and rat NMDAR2A, respectively. (reported region: D958-R1300).
- Molecular weight context: reported MW: 165 kDa; calculated MW: 165283 MW
- Reactivity: Human,Mouse,Rat
- Applications: WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Glutamate receptor ionotropic, NMDA 2A; Glutamate receptor ionotropic, NMDA 2A. GRIN2A is also known as N-methyl-D-aspartate receptor channel, subunit epsilon-1 (NMDAR2A). This gene encodes a member of the glutamate-gated ion channel protein family. The encoded protein is an N-methyl-D-aspartate (NMDA) receptor subunit. NMDA receptors are both ligand-gated and voltage-dependent, and are involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. These receptors are permeable to calcium ions, and activation results in a calcium influx into post-synaptic cells, which results in the activation of several signaling cascades. Disruption of this gene is associated with focal epilepsy and speech disorder with or without mental retardation. Alternative splicing results in multiple transcript variants. Functional note: NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Activation requires binding of agonist to both types of subunits. Reported localization: Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein.
Research relevance and current trends
- Ligand-Gated Ion Channels: Researchers commonly examine how GRIN2A (Glutamate receptor ionotropic, NMDA 2A) relates to this theme using model systems and orthogonal readouts.
- Neuroscience: Researchers commonly examine how GRIN2A (Glutamate receptor ionotropic, NMDA 2A) relates to this theme using model systems and orthogonal readouts.
- Neurotransmission: Researchers commonly examine how GRIN2A (Glutamate receptor ionotropic, NMDA 2A) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative GRIN2A (Glutamate receptor ionotropic, NMDA 2A) levels across conditions; band patterns may reflect isoforms and processing.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
