| Field | Specification |
|---|---|
| Target | |
| Alternative names | Nucleoside diphosphate kinase B; NDP kinase B; NDPK B; C-myc purine-binding transcription factor PUF; Histidine protein kinase NDKB; Nucleoside diphoshate kinase 2; NDK2; nm23-H2; NME2; NM23B |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human NME2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 26N09 is a rabbit monoclonal antibody that recognizes nucleoside diphosphate kinase B (gene NME2) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 17.3 kDa, and the supplier reports an observed band at about 17 kDa.
NME2 (NM23-H2) is nucleoside diphosphate kinase B, an enzyme that generates nucleoside triphosphates from diphosphates using ATP as phosphate donor. Beyond this housekeeping role it can phosphorylate histidine residues on other proteins and bind DNA to influence transcription, including of MYC. It is studied in nucleotide homeostasis, gene regulation and cancer.
| Target | Nucleoside diphosphate kinase B (gene NME2; UniProt P22392, human) |
|---|---|
| Clone | 26N09 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 17.3 kDa |
| Observed MW | 17 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): human LNCAP cells, human 293T cells, human PC-3 cells, human MCF-7 cells, rat liver tissue, rat RH35 cells, mouse liver tissue, mouse HEPA1–6 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 12% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human NME2.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places nucleoside diphosphate kinase B in the cytoplasm, nucleus and cell projection. Tissue expression noted by UniProt (human): Ubiquitously expressed... The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
