| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Neuromedin-U; Neuromedin-U-25; NmU-25; NMU |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human NMU, which shares 64% and 56% amino acid (aa) sequence identity with mouse and rat NMU, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of NMU in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-NMU Antibody Picoband® catalog # A01919-1. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the C-terminus of human NMU, which shares 64% and 56% amino acid (aa) sequence identity with mouse and rat NMU, respectively.
- Molecular weight context: reported MW: 26 kDa; calculated MW: nan
- Reactivity: Mouse,Rat
- Applications: IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
neuromedin U. Neuromedin U (NMU) is a neuropeptide with potent activity on smooth muscle, which was isolated first from porcine spinal cord and later from other species. It is a member of the neuromedin family of neuropeptides. The encoded protein is a precursor that is proteolytically processed to generate a biologically active neuropeptide that plays a role in pain, stress, immune-mediated inflammatory diseases and feeding regulation. Increased expression of this gene was observed in renal, pancreatic and lung cancers. Alternative splicing results in multiple transcript variants encoding different isoforms. Some of these isoforms may undergo similar processing to generate the mature peptide. Functional note: Stimulates muscle contractions of specific regions of the gastrointestinal tract. In humans, NmU stimulates contractions of the ileum and urinary bladder. Reported localization: Secreted. Expression/tissue context: Expressed throughout the enteric nervous system with highest levels being found in the jejunum.
Research relevance and current trends
- Neuropeptides: Researchers commonly examine how NMU relates to this theme using model systems and orthogonal readouts.
- Neuroscience: Researchers commonly examine how NMU relates to this theme using model systems and orthogonal readouts.
- Neurotransmitter: Researchers commonly examine how NMU relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative NMU levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of NMU across tissue regions and cell types using matched controls.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
