| Field | Specification |
|---|---|
| Target | |
| Alternative names | Neurogenic locus notch homolog protein 1; Notch 1; hN1; Translocation-associated notch protein TAN-1; NOTCH1; TAN1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Notch1. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone BGA-14 is a rabbit monoclonal antibody that recognizes neurogenic locus notch homolog protein 1 (gene NOTCH1) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 272.5 kDa, and the supplier reports an observed band at about 110 kDa.
NOTCH1 is a single-pass transmembrane receptor activated by Jagged and Delta-like ligands on neighboring cells. Ligand binding triggers cleavage that releases the Notch intracellular domain (NICD), which moves to the nucleus and activates target genes with RBPJ. NOTCH1 controls cell fate in many tissues, including T-cell development, and is frequently mutated in T-cell acute lymphoblastic leukemia.
| Target | Neurogenic locus notch homolog protein 1 (gene NOTCH1; UniProt P46531, human) |
|---|---|
| Clone | BGA-14 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 272.5 kDa |
| Observed MW | 110 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): human 293T cells, human K562 cells, human Jurkat cells, rat brain tissue, rat PC-12 cells, mouse brain tissue, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human liver tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human notch1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places neurogenic locus notch homolog protein 1 in the cell membrane and late endosome membrane. Tissue expression noted by UniProt (human): In fetal tissues most abundant in spleen, brain stem and lung. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yuan Li et al. (2022) Establishment of an astrocyte-like cell line from the brain of tilapia (Oreochromis niloticus) for virus pathogenesis and a vitro model of the blood–brain barrier. Journal of Fish Diseases. 10.1111/jfd.13674
Chun Li et al. (2021) FOXP3 facilitates the invasion and metastasis of non‑small cell lung cancer cells through regulating VEGF, EMT and the Notch1/Hes1 pathway. Experimental and Therapeutic Medicine. 10.3892/etm.2021.10390
