| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Syntenin-1; Melanoma differentiation-associated protein 9; MDA-9; Pro-TGF-alpha cytoplasmic domain-interacting protein 18; TACIP18; Scaffold protein Pbp1; Syndecan-binding protein 1; SDCBP; MDA9; SYCL |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human NOXA2/p67phox/NCF2 recombinant protein (Position: M1-V526). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-NOXA2/p67phox/NCF2 Antibody Picoband® is an antibody for NCF2 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: NCF2 (syndecan binding protein); UniProt: P19878
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 60 kDa, calculated 22105 MW
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-NOXA2/p67phox/NCF2 Antibody Picoband® catalog # A02476-1.
Biological background
Biological context: Multifunctional adapter protein involved in diverse array of functions including trafficking of transmembrane proteins, neuro and immunomodulation, exosome biogenesis, and tumorigenesis. Positively regulates TGFB1-mediated SMAD2/3 activation and TGFB1-induced epithelial-to-mesenchymal transition (EMT) and cell migration in various cell types. May increase TGFB1 signaling by enhancing cell-surface expression of TGFR1 by preventing the interaction between TGFR1 and CAV1 and subsequent CAV1-dependent internalization and degradation of TGFR1. In concert with SDC1/4 and PDCD6IP, regulates exosome biogenesis. Regulates migration, growth, proliferation, and cell cycle progression in a variety of cancer types. In adherens junctions may function to couple syndecans to cytoskeletal proteins or signaling components. Seems to couple transcription factor SOX4 to the IL-5 receptor (IL5RA). May also play a role in vesicular trafficking. Seems to be required for the targeting of TGFA to the cell surface in the early secretory pathway.
Expression and localization notes: cellular localization: Nucleus. Exosome. Endoplasmic reticulum membrane. Peripheral membrane protein. Cytosol. Cytoskeleton. Cell membrane. Peripheral membrane protein., tissue context: Expressed in lung cancers, including adenocarcinoma, squamous cell carcinoma and small-cell carcinoma. Widely expressed. Expressed in fetal kidney, liver, lung and brain. In adult highest expression in heart and placenta..
Common research applications
- Western blotting (WB): Compare NCF2 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of NCF2 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify NCF2-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: This gene encodes neutrophil cytosolic factor 2, the 67-kilodalton cytosolic subunit of the multi-protein NADPH oxidase complex found in neutrophils. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Mutations in this gene, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease, a disease that causes recurrent infections by catalase-positive organisms. Alternative splicing results in multiple transcript variants encoding different isoforms.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Nucleus. Exosome. Endoplasmic reticulum membrane. Peripheral membrane protein. Cytosol. Cytoskeleton. Cell membrane. Peripheral membrane protein.
- Tissue details: Expressed in lung cancers, including adenocarcinoma, squamous cell carcinoma and small-cell carcinoma. Widely expressed. Expressed in fetal kidney, liver, lung and brain. In adult highest expression in heart and placenta.
- Research category: Cell Type Marker,Neurogenesis,Neurology Process,Neuron Marker,Neuroscience,Synapse Marker
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
