| Field | Specification |
|---|---|
| Target | |
| Alternative names | NPRL2; TUSC4; GATOR complex protein NPRL2; Gene 21 protein; G21 protein; Nitrogen permease regulator 2-like protein; NPR2-like protein; Tumor suppressor candidate 4 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human NPRL2 recombinant protein (Position: D90-K380). Human NPRL2 shares 98.3% amino acid (aa) sequence identity with mouse NPRL2. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects GATOR1 complex protein NPRL2 in mouse and rat samples and is validated for Western blot, flow cytometry and ELISA.
NPRL2 is a subunit of GATOR1, a complex with NPRL3 and DEPDC5 that acts as a GTPase-activating protein for RagA/B. When amino acids are scarce, GATOR1 turns off Rag signaling and so inhibits mTORC1. NPRL2 is studied in nutrient sensing and mTOR regulation, and GATOR1 mutations are associated with familial focal epilepsy.
| Target | GATOR1 complex protein NPRL2 (gene NPRL2; UniProt Q8WTW4, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Immunogen
Recombinant human NPRL2 fragment (Asp90–Lys380), expressed in E. coli. Sequence identity with the mouse ortholog: 98.3%.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places GATOR1 complex protein NPRL2 in the lysosome membrane. Tissue expression noted by UniProt (human): Most abundant in skeletal muscle, followed by brain, liver and pancreas, with lower amounts in lung, kidney, placenta and heart.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
