| Field | Specification |
|---|---|
| Target | |
| Alternative names | Neuropeptide Y receptor type 5; NPY5-R; NPY-Y5 receptor; NPYY5-R; Y5 receptor; NPY5R; NPYR5 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human NPY5R |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 27N62 is a rabbit monoclonal antibody that recognizes neuropeptide Y receptor type 5 (gene NPY5R) in human samples and is validated for Western blot, ICC/IF and flow cytometry. The predicted molecular weight is 50.7 kDa, and the supplier reports an observed band at about 51 kDa.
Neuropeptide Y receptor Y5 (NPY5R) is a G protein-coupled receptor for NPY and peptide YY that inhibits adenylyl cyclase. It is expressed in hypothalamic regions that regulate feeding and has been studied as a mediator of NPY-driven food intake and energy balance.
| Target | Neuropeptide Y receptor type 5 (gene NPY5R; UniProt Q15761, human) |
|---|---|
| Clone | 27N62 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 50.7 kDa |
| Observed MW | 51 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): Ramos cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human NPY5R.
Reactivity Notes
The supplier lists reactivity with human. UniProt places neuropeptide Y receptor type 5 in the cell membrane. Tissue expression noted by UniProt (human): Brain. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
