| Field | Specification |
|---|---|
| Target | |
| Alternative names | Nuclear receptor subfamily 2 group F member 6;V-erbA-related protein 2;EAR-2;NR2F6;EAR2, ERBAL2; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human NR2F6 recombinant protein (Position: M270-Q404). Human NR2F6 shares 99.3% amino acid (aa) sequence identity with both mouse and rat NR2F6. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets nuclear receptor subfamily 2 group F member 6 (gene NR2F6) in human and rat samples and is validated for Western blot. The predicted molecular weight is 43.0 kDa, and the supplier reports an observed band at about 43 kDa.
NR2F6 (also called EAR2) is an orphan nuclear receptor of the COUP-TF family that mainly represses transcription. In T cells it limits production of cytokines such as IL-2, IL-17 and IFN-γ, acting as an intracellular immune checkpoint. It is studied in T-cell regulation, autoimmunity and antitumor immunity.
| Target | Nuclear receptor subfamily 2 group F member 6 (gene NR2F6; UniProt P10588, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 43.0 kDa |
| Observed MW | 43 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Rat) |
|---|
Samples with a confirmed band (WB): rat liver tissue, HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human NR2F6 fragment (Met270–Gln404), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 99.3%.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places nuclear receptor subfamily 2 group F member 6 in the nucleus. Tissue expression noted by UniProt (human): Expressed in heart, placenta, liver, skeletal muscle, kidney and pancreas. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
