Anti-NSD3/WHSC1L1 Rabbit Monoclonal Antibody

Overview

Anti-NSD3/WHSC1L1 Rabbit Monoclonal Antibody is an antibody targeting WHSC1L1. Common applications include WB, Flow Cytometry. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: FFH-23; isotype: Rabbit IgG; reactivity: Human,Mouse,Rat; observed MW: 95 kDa; calculated MW: 161613 MW.

Boster Bio Anti-NSD3/WHSC1L1 Rabbit Monoclonal Antibody catalog # M05608. Tested in WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Key elements and design rationale

• Target: WHSC1L1 — Histone-lysine N-methyltransferase NSD3
• Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: FFH-23; Isotype: Rabbit IgG
• Species reactivity: Human,Mouse,Rat
• Molecular weight guidance: Observed: 95 kDa; Calculated: 161613 MW

Biological background

Protein function (datasheet): Histone methyltransferase. Preferentially methylates 'Lys-4' and 'Lys-27' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation, while 'Lys-27' is a mark for transcriptional repression. .

Cellular localization (datasheet): Nucleus . Chromosome .

Tissue details (datasheet): Highly expressed in brain, heart and skeletal muscle. Expressed at lower level in liver and lung. .

Research relevance and current trends

• Commonly studied in contexts related to 2339,Developmental Biology,Embryogenesis,Epigenetics and Nuclear Signaling,Lineage Markers,Lineage Specification,Nervous System Development,Neurogenesis,Neurology Process,Neuroscience,Organogenesis,Sensory System,Stem Cells,Transcription,Visual System.
• Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
• Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).

Common research applications

• Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
• Flow cytometry: Quantify target-positive populations in single-cell suspensions; pair with viability and isotype/FMO controls conceptually.

Notes for experimental interpretation

• Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
• Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.

As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.