| Field | Specification |
|---|---|
| Target | |
| Alternative names | RNA cytosine C; 5; -methyltransferase NSUN2; Myc-induced SUN domain-containing protein; Misu; NOL1/NOP2/Sun domain family member 2; Substrate of AIM1/Aurora kinase B; mRNA cytosine C(5)-methyltransferase; tRNA cytosine C(5)-methyltransferase; EC 2.1.1.-, EC 2.1.1.203; tRNA methyltransferase 4 homolog; hTrm4; NSUN2; SAKI; TRM4 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human NSUN2 recombinant protein (Position: H146-K654). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets RNA cytosine C(5)-methyltransferase NSUN2 in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 86.5 kDa, and the supplier reports an observed band at about 100 kDa.
NSUN2 is an RNA methyltransferase that converts cytosine to 5-methylcytosine (m5C) in tRNAs, mRNAs and some non-coding RNAs. The modification affects tRNA stability and protein synthesis and helps control stem cell differentiation. NSUN2 loss causes a recessive intellectual disability syndrome, and it is studied in RNA modification and cancer.
| Target | RNA cytosine C(5)-methyltransferase NSUN2 (gene NSUN2; UniProt Q08J23, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 86.5 kDa |
| Observed MW | 100 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 1–2 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Rat) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HeLa cells, human 293T cells, rat brain tissue, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human acinic cells carcinoma tissue, human colorectal adenocarcinoma tissue, human liver cancer tissue, human lung cancer tissue, human ovarian cancer tissue, human tonsil tissue, human urothelial carcinoma tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed signal (flow cytometry): 293T cells.
Samples with confirmed staining (ICC/IF): NRK cells.
Immunogen
Recombinant human NSUN2 fragment (His146–Lys654), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes RNA cytosine C(5)-methyltransferase NSUN2 as secreted and also located in the nucleus, cytoplasm and mitochondrion. Tissue expression noted by UniProt (human): Expressed in adult and fetal brain and in lymphoblastoid cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
