| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Nucleolysin TIA-1 isoform p40;RNA-binding protein TIA-1;T-cell-restricted intracellular antigen-1;TIA-1;p40-TIA-1;TIA1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human TIA1, identical to the related mouse sequence. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Nucleolysin TIA-1 isoform p40 TIA1 Antibody Picoband® is an antibody targeting TIA1. Common applications include WB, IHC, Flow Cytometry, ELISA. Key specifications include host: Rabbit; clonality: Polyclonal; isotype: Rabbit IgG; reactivity: Mouse,Human; observed MW: 274 kDa; calculated MW: 42963 MW.
Boster Bio Anti-Nucleolysin TIA-1 isoform p40 TIA1 Antibody catalog # PA2194. Tested in WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Target: TIA1 — Nucleolysin TIA-1 isoform p40
- Antibody format: Host: Rabbit; Clonality: Polyclonal; Isotype: Rabbit IgG
- Species reactivity: Mouse,Human
- Molecular weight guidance: Observed: 274 kDa; Calculated: 42963 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): Involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis.
Scientific background (datasheet): TIA1, also called WDM, encodes an RNA-binding protein involved in splicing regulation and translational repression. The product encoded by this gene is a member of a RNA-binding protein family and possesses nucleolytic activity against cytotoxic lymphocyte (CTL) target cells. By in situ hybridization, this gene is mapped to chromosome 2p13.3. It has been suggested that this protein may be involved in the induction of apoptosis as it preferentially recognizes poly (A) homopolymers and induces DNA fragmentation in CTL targets. The major granule-associated species is a 15-kDa protein that is though to be derived from the carboxyl terminus of the 40-kDa product by proteolytic processing.
Cellular localization (datasheet): Cytoplasmic granule. Nucleus. Accumulates in cytoplasmic stress granules (SG) following cellular damage.
Tissue details (datasheet): Expressed mostly in brain, muscle, and pancreas but detected in all tissues examined.
Sequence similarities (datasheet): Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
Research relevance and current trends
- Commonly studied in contexts related to Adaptive Immunity,Apoptosis,Cancer,Cell Biology,Cell Death,DNA/RNA,Epigenetics and Nuclear Signaling,Immunology,Non-CD,Nucleus,RNA Processing,T Cells.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- ELISA: Measure target abundance in compatible matrices using a standard-curve readout; ensure dilution linearity and appropriate controls.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
- Flow cytometry: Quantify target-positive populations in single-cell suspensions; pair with viability and isotype/FMO controls conceptually.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a polyclonal antibody, this reagent may recognize multiple epitopes on the target, which can improve detection robustness but may require careful specificity controls.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
