Anti-NUDC Rabbit Monoclonal Antibody

SKU:BHA21009738
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Suppliers
Boster Bio
Boster Bio
Details Products
Overview
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Anti-NUDC antibody (Rabbit monoclonal, clone 22N41, IgG). Validated for Western blot, immunohistochemistry, immunofluorescence, immunoprecipitation and flow cytometry. Reactivity: Human, Mouse, Rat. Recognizes nuclear migration protein nudC.
Target NUDC
clone number 22N41
Host Rabbit
Reactivity Human,Mouse,Rat
Isotype IgG
Application(s) WB, IHC, ICC, IF, IP, Flow
Options selector
Catalog no. Size Conjugation
M00995 100 uL/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • Size: 100 uL/vial; Conjugation: Unconjugated
      Form: Liquid
      Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
      Applications: WB,IHC,ICC,IF,IP,Flow Cytometry
      Application details: WB 1:500-2000<br>IHC 1:50-200<br>ICC/IF 1:50-200<br>IP 1:50<br>FC 1:50<br>
      Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
  • Lead time: typically ships in ~2-3 business days; timing may vary by selected option.
  • Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target NUDC
Alternative names Nuclear migration protein nudC; Nuclear distribution protein C homolog; NUDC
UniProt # Q9Y266
Host Rabbit
Clone 22N41
Clonality
  • Monoclonal
Isotype
  • IgG
Reactivity
  • Human
  • Mouse
  • Rat
Applications
  • Western Blot
  • Immunohistochemistry
  • Immunofluorescence
  • Immunoprecipitation
  • Flow Cytometry
Immunogen A synthesized peptide derived from human NUDC
Molecular weight 38.2 kDa
Purification Affinity-chromatography
Reconstitution Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Cellular localization Cytoplasm . Nucleus . In response to stress, recruited by p53/TP53 to specific promoters.
Concentration 0.5mg/ml
Form Liquid
Storage Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) M00995
Main SKU BHA21009738
Rabbit Monoclonal

Product Overview

Clone 22N41 is a rabbit monoclonal antibody that recognizes nuclear migration protein nudC in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 38.2 kDa, and the supplier reports an observed band at about 42 kDa.

NudC (nuclear distribution protein C) is a conserved protein that works with the dynein–LIS1 motor system. It is needed for proper mitotic spindle formation, chromosome separation and cytokinesis, and it also contributes to neuronal migration during brain development.

Target Nuclear migration protein nudC (gene NUDC; UniProt Q9Y266, human)
Clone 22N41
Host / Clonality / Isotype Rabbit / Monoclonal / IgG
Reactivity Human, Mouse, Rat
Form Liquid
Formulation PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers
Calculated MW 38.2 kDa
Observed MW 42 kDa
Storage Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Validated Applications

Western blot 1:500–1:2000
Immunohistochemistry 1:50–1:200
Immunocytochemistry / Immunofluorescence 1:50–1:200
Immunoprecipitation 1:50
Flow cytometry 1:50

Samples with a confirmed band (WB): HeLa cells.

Recommended loading (WB): 20–40 µg of total protein per lane.

Immunogen

Synthetic peptide based on the sequence of human NUDC.

Reactivity Notes

The supplier lists reactivity with human, mouse and rat. UniProt places nuclear migration protein nudC in the cytoplasm, nucleus and midbody. Tissue expression noted by UniProt (human): Ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: Western blot 1:500–1:2000; Immunohistochemistry 1:50–1:200; Immunocytochemistry / Immunofluorescence 1:50–1:200; Flow cytometry 1:50; Immunoprecipitation 1:50. Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Human, Mouse, Rat. Other species are not listed by the supplier.
Q.What band size should be expected in Western blot?
A.Reported by the supplier: calculated molecular weight 38.2 kDa; observed band at about 42 kDa.
Q.What immunogen was used to raise this antibody?
A.Synthetic peptide based on the sequence of human NUDC.
Q.How should this antibody be stored?
A.Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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