| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human NUDT19 recombinant protein (Position: H52-L375). Human NUDT19 shares 66.4% and 65.8% amino acid (aa) sequence identity with mouse and rat NUDT19, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-NUDT19 Antibody Picoband® is an antibody reagent for detection of NUDT19. Researchers commonly use anti-NUDT19 antibodies to measure relative expression and localization across biological samples, with assay selection guided by the listed applications (WB, IHC, Flow, ELISA).
Boster Bio Anti-NUDT19 Antibody Picoband® catalog # A14864-1. Tested in WB, IHC, Flow Cytometry, ELISA applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Target: NUDT19
- Antibody format: Polyclonal; IgG
- Species context: Host: Rabbit, Reactivity: Human,Rat
- Purification: Immunogen affinity purified.
- Immunogen: E.coli-derived human NUDT19 recombinant protein (Position: H52-L375). Human NUDT19 shares 66.4% and 65.8% amino acid (aa) sequence identity with mouse and rat NUDT19, respectively.
- Molecular weight context: observed 40 kDa (reported)
- Provided application(s): WB, IHC, Flow, ELISA
These attributes help contextualize how the antibody is commonly selected (host/clonality/isotype/label) and how signals are interpreted across sample types and assay formats.
Biological background
NUDT19 is commonly studied as part of broader cellular pathways and regulatory networks. Expression level, localization, and isoform context can vary by cell type, state, and stimulus, so interpretation typically considers biological context alongside assay controls.
Background: NUDT19 (nudix (nucleoside diphosphate linked moiety X)-type motif 19), also known as RP2, is a 375 amino acid protein and coenzyme A diphosphatase that assists in the hydrolysis of CoA esters. A member of the nudix hydrolase family, NUDT19 contains one nudix hydrolase domain and localizes to both mitochondria and peroxisome. NUDT19 utilizes magnesium and/or manganese as cofactors and is encoded by a gene that maps to human chromosome 19q13.11. Chromosome 19 consists of over 63 million bases, houses approximately 1,400 genes and is recognized for having the greatest gene density of the human chromosomes.
Research relevance and current trends
- Quantitative and spatial profiling: expression patterns are increasingly studied across cell states using multiplex imaging and omics-informed validation.
- Isoforms and post-translational modifications: researchers often evaluate how isoform composition and PTMs can shift apparent molecular weight or localization.
- Context-aware interpretation: comparative studies commonly include perturbations (stimulation, inhibition, genetic models) to relate target changes to pathway behavior.
Common research applications
- Western blot (WB): compare relative target abundance and apparent size shifts (e.g., isoforms/PTMs) across conditions.
- Immunohistochemistry (IHC): assess distribution across tissue compartments and compare staining patterns between groups.
- Flow cytometry: quantify target-positive populations and compare shifts after stimulation or differentiation.
Across these uses, researchers typically interpret changes in signal as relative differences between matched sample groups, considering sample preparation and biological context.
Notes for experimental interpretation
- Apparent molecular weight can vary due to isoforms, proteolysis, glycosylation, phosphorylation, and sample preparation differences.
- Species reactivity and epitope conservation can influence observed signal patterns, especially in cross-species studies.
- Control concepts: include appropriate negative controls (e.g., isotype controls where relevant) and, when feasible, genetic or orthogonal controls (KO/KD, peptide competition, or independent assays) to support interpretation.
For antibody reagents, monoclonal antibodies are often chosen for epitope consistency across lots, while polyclonals may recognize multiple epitopes and can show different background characteristics depending on context.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
