| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Histone deacetylase 9;HD9;3.5.1.98;Histone deacetylase 7B;HD7;HD7b;Histone deacetylase-related protein;MEF2-interacting transcription repressor MITR;HDAC9;HDAC7, HDAC7B, HDRP, KIAA0744, MITR; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Gene ID | |
| Host | |
| Immunogen | A synthesized peptide derived from human Nup153 Possible DNA-binding subunit of the nuclear pore complex (NPC) . The repeat-containing domain may be involved in anchoring components of the pore complex to the pore membrane. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-NUP153 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone AFDA-14; isotype Rabbit IgG; reactivity: Human. Reported application contexts include WB, ICC, IF, Flow (as provided in the source record). Boster Bio Anti-NUP153 Monoclonal Antibody catalog # M02183. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Key elements and design rationale
- Target: NUP153 (Histone deacetylase 9).
- Antibody format: Monoclonal; clone AFDA-14; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
NUP153 (protein: Lysosome-associated membrane glycoprotein 2 (Lamp2)) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Represses MEF2-dependent transcription. Reported cellular localization context: Nucleus . Tissue expression notes (as provided): Broadly expressed, with highest levels in brain, heart, muscle and testis. Isoform 3 is present in human bladder carcinoma cells (at protein level). .
Research relevance and current trends
- Research context keywords from the source record include: Epigenetics and Nuclear Signaling,Nuclear Import/Export,Nuclear Receptors,Nuclear Signaling Pathways,Nucleus,Protein Trafficking,Signal Transduction,Subcellular Markers,Tags & Cell Markers.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
Workflow ideas (metafield): Validate NUP153 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect NUP153 expression by Western blot in cell or tissue lysates, Localize NUP153 by immunofluorescence/immunocytochemistry in cultured cells, Quantify NUP153-positive cells by flow cytometry in single-cell suspensions
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 27 kDa; calculated MW: 111297 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 27 kDa
- Cellular localization (provided): Nucleus .
- Tissue details (provided): Broadly expressed, with highest levels in brain, heart, muscle and testis. Isoform 3 is present in human bladder carcinoma cells (at protein level). .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
