| Field | Specification |
|---|---|
| Target | |
| Alternative names | N-glycosylase/DNA lyase; 8-oxoguanine DNA glycosylase; DNA- (apurinic or apyrimidinic site) lyase; AP lyase; OGG1; MMH; MUTM; OGH1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human Ogg1, which shares 89.7% and 87.2% amino acid (aa) sequence identity with mouse and rat Ogg1, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects N-glycosylase/DNA lyase (gene OGG1) in human samples and is validated for immunoprecipitation, ICC/IF, IHC and Western blot. The predicted molecular weight is 38.8 kDa, and the supplier reports an observed band at about 39 kDa.
OGG1 (8-oxoguanine DNA glycosylase) is the main enzyme that removes 8-oxoguanine, a common oxidative lesion, from DNA. It excises the damaged base and nicks the DNA strand to begin base excision repair, so it is widely studied in oxidative stress, mutagenesis, aging and inflammation.
| Target | N-glycosylase/DNA lyase (gene OGG1; UniProt O15527, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 38.8 kDa |
| Observed MW | 39 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Immunoprecipitation | 0.5–2 µg/mL (Human) |
Samples with a confirmed band (WB): human A549 cells, human HepG2 cells, human HeLa cells, human 293T cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human breast cancer tissue, human lung cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): HeLa cells.
Samples with a confirmed result (IP): 293T cells.
Immunogen
Synthetic peptide from the N-terminal region of human ogg1. Sequence identity with the mouse and rat orthologs: 89.7% and 87.2%, respectively.
Reactivity Notes
The supplier lists reactivity with human. UniProt places N-glycosylase/DNA lyase in the nucleus, nucleus speckle and nucleus matrix. Tissue expression noted by UniProt (human): Ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Hong-qiang Chen et al. (2020) Epigenetic silencing of TET1 mediated hydroxymethylation of base excision repair pathway during lung carcinogenesis. Environmental Pollution. 10.1016/j.envpol.2020.115860
