| Field | Specification |
|---|---|
| Target | |
| Alternative names | Osteocalcin;Bone Gla protein;BGP;Gamma-carboxyglutamic acid-containing protein;BGLAP; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human Osteocalcin recombinant protein (Position: Y52-V100). Human Osteocalcin shares 69% and 80.9% amino acid (aa) sequence identity with mouse and rat Osteocalcin, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects osteocalcin (gene BGLAP) in human samples and is validated for IHC. The predicted molecular weight is 11.0 kDa.
Osteocalcin (BGLAP) is a small bone protein produced by osteoblasts that becomes γ-carboxylated in a vitamin K-dependent manner, allowing it to bind calcium and hydroxyapatite in bone matrix. It is one of the most abundant noncollagenous proteins of bone, and circulating osteocalcin is widely used as a marker of bone formation.
| Target | Osteocalcin (gene BGLAP; UniProt P02818, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 11.0 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Human) |
|---|
Samples with confirmed staining (IHC): human osteosarcoma tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human Osteocalcin fragment (Tyr52–Val100), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 69% and 80.9%, respectively.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes osteocalcin as a secreted protein.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Tongmeng Jiang et al. (2018) Untangling the response of bone tumor cells and bone forming cells to matrix stiffness and adhesion ligand density by means of hydrogels. Biomaterials. 10.1016/j.biomaterials.2018.10.015
Yang Wang et al. (2013) Induced apoptosis of osteoblasts proliferating on polyhydroxyalkanoates. Biomaterials. 10.1016/j.biomaterials.2013.01.088
YiQiang Li et al. (2017) mTORC1 signaling is essential for neurofibromatosis type I gene modulated osteogenic differentiation of BMSCs. Journal of Cellular Biochemistry. 10.1002/jcb.26626
