| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Osteocalcin;Bone Gla protein;BGP;Gamma-carboxyglutamic acid-containing protein;BGLAP; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived human Osteocalcin recombinant protein (Position: Y52-V100). Human Osteocalcin shares 69% and 80.9% amino acid (aa) sequence identity with mouse and rat Osteocalcin, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of BGLAP (Osteocalcin) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Osteocalcin/BGLAP Antibody Picoband® catalog # PB9918. Tested in IHC applications. This antibody reacts with Human.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived human Osteocalcin recombinant protein (Position: Y52-V100). Human Osteocalcin shares 69% and 80.9% amino acid (aa) sequence identity with mouse and rat Osteocalcin, respectively. (reported region: Y52-V100).
- Molecular weight context: reported MW: 29 kDa, 60 kDa; calculated MW: 10963 MW
- Reactivity: Human
- Applications: IHC
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Osteocalcin; Osteocalcin. Osteocalcin, also known as bone gamma-carboxyglutamic acid-containing protein (BGLAP), is a noncollagenous protein found in bone and dentin. In humans, the osteocalcin is encoded by the BGLAP gene. Its receptor is GPRC6A. It is mapped to 1q22. Osteocalcin may play a role in the body's metabolic regulation and is pro-osteoblastic, or bone-building. It acts as a hormone in the body, causing beta cellsin the pancreas to release more insulin, and at the same time ing fat cells to release the hormone adiponectin, which increases sensitivity to insulin. Also, it may play a role in male fertility. And it is found that picomolar amounts of osteocalcin affected insulin secretion and beta-cell proliferation. Functional note: Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. Reported localization: Secreted. Expression/tissue context: Bcl-X (S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X (L) is found in tissues containing long-lived postmitotic cells, such as adult brain.
Research relevance and current trends
- Cytoskeleton/ECM: Researchers commonly examine how BGLAP (Osteocalcin) relates to this theme using model systems and orthogonal readouts.
- Extracellular Matrix: Researchers commonly examine how BGLAP (Osteocalcin) relates to this theme using model systems and orthogonal readouts.
- Mesenchymal Stem Cells: Researchers commonly examine how BGLAP (Osteocalcin) relates to this theme using model systems and orthogonal readouts.
Common research applications
- IHC/IHC-F: assess spatial distribution of BGLAP (Osteocalcin) across tissue regions and cell types using matched controls.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
