| Field | Specification |
|---|---|
| Target | |
| Alternative names | Osteopontin;Bone sialoprotein 1;Nephropontin;Secreted phosphoprotein 1;SPP-1;Urinary stone protein;Uropontin;SPP1;BNSP, OPN;PSEC0156; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human Osteopontin, different from the related mouse sequence by eight amino acids, and from the related rat sequence by seven amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes osteopontin (gene SPP1) in human, mouse and rat samples and is validated for ELISA and Western blot. The predicted molecular weight is 35.4 kDa; the supplier reports observed bands at 66 kDa, 25–55 kDa.
Osteopontin (SPP1) is a secreted, phosphorylated glycoprotein first identified as a major non-collagenous protein of bone, where it binds hydroxyapatite and mediates cell-matrix interactions. It also acts as a cytokine that promotes interferon-γ and IL-12 production, and it is widely studied in bone remodeling, inflammation, fibrosis and tumor progression.
| Target | Osteopontin (gene SPP1; UniProt P10451, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 35.4 kDa |
| Observed MW | 66 kDa, 25–55 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): rat brain tissue, mouse brain tissue, human SHG-44 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
Synthetic peptide from the C-terminal region of human osteopontin. It differs from the mouse sequence at eight residues and from the rat sequence at seven residues.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes osteopontin as a secreted protein. Tissue expression noted by UniProt (human): Detected in cerebrospinal fluid and urine (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Haiyan Yao et al. (2025) L-arginine modified mesoporous bioactive glass with ROS scavenging and NO release for periodontitis treatment. Bioactive Materials. 10.1016/j.bioactmat.2025.02.015
Shuangqin Chen et al. (2022) β-catenin-controlled tubular cell-derived exosomes play a key role in fibroblast activation via the OPN-CD44 axis. Journal of Extracellular Vesicles. 10.1002/jev2.12203
Fei Long et al. (2024) DAB2+ macrophages support FAP+ fibroblasts in shaping tumor barrier and inducing poor clinical outcomes in liver cancer. Theranostics. 10.7150/thno.99046
Fei Chengshuo et al. (2025) Single-cell multi-omics analysis identifies SPP1+ macrophages as key drivers of ferroptosis-mediated fibrosis in ligamentum flavum hypertrophy. Biomarker Research. 10.1186/s40364-025-00746-6
Bailong Tao et al. (2020) Surface modification of titanium implants by ZIF-8@Levo/LBL coating for inhibition of bacterial-associated infection and enhancement of in vivo osseointegration. Chemical Engineering Journal. 10.1016/j.cej.2020.124621
Caiyun Mu et al. (2020) Construction of chemokine substance P-embedded biomimetic multilayer onto bioactive magnesium silicate-titanium implant for bone regeneration. Applied Materials Today. 10.1016/j.apmt.2020.100777
Zhaozhao Ding et al. (2017) Nanoscale Silk–Hydroxyapatite Hydrogels for Injectable Bone Biomaterials. ACS Applied Materials & Interfaces. 10.1021/acsami.7b03932
Yang Xun et al. (2022) Role of Nox4 in High Calcium-Induced Renal Oxidative Stress Damage and Crystal Deposition. Antioxidants & Redox Signaling. 10.1089/ars.2020.8159
Weizhen Chen et al. (2018) Multilayered coating of titanium implants promotes coupled osteogenesis and angiogenesis in vitro and in vivo. Acta Biomaterialia. 10.1016/j.actbio.2018.04.043
Wentao Shi et al. (2022) A mussel-bioinspired coating strategy to licence PCL osteoinductive activity for repairing bone defects. Journal of Materials Research and Technology-JMR&T. 10.1016/j.jmrt.2022.03.064
