| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Homeobox protein OTX1;Orthodenticle homolog 1;OTX1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Gene ID | |
| Host | |
| Immunogen | A synthesized peptide derived from human Otx1/2 Probably plays a role in the development of the brain and the sense organs. Can bind to the BCD target sequence (BTS) : 5'-TCTAATCCC-3'. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-OTX1 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone ADBB-15; isotype Rabbit IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, IHC (as provided in the source record). Boster Bio Anti-Otx1/2 Monoclonal Antibody catalog # M05601-1. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: OTX1 (Homeobox protein OTX1).
- Antibody format: Monoclonal; clone ADBB-15; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
OTX1 (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Probably plays a role in the development of the brain and the sense organs. Can bind to the BCD target sequence (BTS): 5'-TCTAATCCC-3'. Reported cellular localization context: Nucleus . Tissue expression notes (as provided): Expressed in brain. Detected in the anterior part of the neural fetal retina (at protein level). .
Research relevance and current trends
- Research context keywords from the source record include: 2339,Developmental Biology,Embryogenesis,Epigenetics and Nuclear Signaling,Lineage Markers,Lineage Specification,Nervous System Development,Neurogenesis,Neurology Process,Neuroscience,Organogenesis,Sensory System,Stem Cells,Transcription,Visual System.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
Workflow ideas (metafield): Validate OTX1 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect OTX1 expression by Western blot in cell or tissue lysates, Detect OTX1 in FFPE tissue sections by immunohistochemistry
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 95 kDa; calculated MW: 37327 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 95 kDa
- Cellular localization (provided): Nucleus .
- Tissue details (provided): Expressed in brain. Detected in the anterior part of the neural fetal retina (at protein level). .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
