| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | tumor necrosis factor ligand superfamily member 4, TNFSF4, CD252, TNF Receptor Superfamily Member 4, TRAF2 |
| Clonality | |
| Conjugate | |
| Formulation | |
| Host | |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Shipping | |
| Species | |
| Storage | |
| Target | |
| UniProt # |
Immunotherapy
Product Overview
Recombinant human Anti-OX40 competitive antibody recognizing the OX40 binding region. This antibody has not been tested for cross-reactivity with any other species.
| Clonality | Monoclonal |
|---|---|
| Host | Human |
| Antibody Type | IgG1 |
| Isotype | IgG1 |
| Reactivity | Human |
| Molecular Weight | Heavy Chain: 51 kDa, Light Chain: 26 kDa |
| Formulation | 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, pH 7.4, and 20% glycerol |
| UniProt | P23510 |
| Storage | Stable for at least 12 months at -80°C. |
Validated Applications
- Western Blot
Assay conditions: Reaction done according to OX40[biotin]-OX40L assay kit (Catalog # 72045). Buffer is PBS + 0.1% BSA. Blocking buffer is Superblock + 0.05% Tween-20. Coat protein was added ON at 4 degrees. Inhibitor and then biotinylated ligand added for 2 hours at room temperature. Strep-HRP added for 1 hour at room temperature.
Immunogen / Antigen Information
Immunogen information not provided. Please refer to the product datasheet for detailed antigen information.
Cross-Reactivity & Specificity
Cross-reactivity data: Species reactivity as listed. No additional cross-reactivity data is available from the manufacturer.
Safety & Handling
Protect from light. Avoid freeze/thaw cycles. Store diluted antibody with carrier protein (BSA 0.1 - 0.5%) at 4°C, stable for 2 weeks.
For Research Use Only (RUO). Not for diagnostic, therapeutic, or clinical use. Handle with standard laboratory precautions (gloves, safety glasses, lab coat). Avoid freeze-thaw cycles. Consult SDS prior to use.
Related Products
- 71175
- 71185
- 72063-1
- 72063-2
- 72064-2
- 72064-1
- 60682
This antibody has been validated for: Western Blot. Optimal dilutions and concentrations should be empirically determined for each specific application and sample type. Refer to the product datasheet for suggested starting conditions.
This antibody should be stored at the indicated temperature to maintain stability. Store at Stable for at least 12 months at -80°C.. Avoid repeated freeze-thaw cycles, which can reduce antibody performance and increase aggregation. Centrifuge briefly after thawing and keep on ice during use.
Please refer to the BPS Bioscience datasheet for detailed immunogen information.
Cross-reactivity data is limited; refer to the product datasheet for available information.
BPS Bioscience offers multiple antibody formats for key checkpoint and immunotherapy targets. Contact BioHippo (support@biohippo.com) or click "Talk to a Scientist" to inquire about additional conjugate formats, bulk quantities, or custom formulations.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
- Garrison, K., et al., Cancer Immunology. 2012 April; 61(4): 511-521.
- Bulliard, Y., et al., Immunology & Cell Biology. 2014 Apr; 92: 475-480.