| Field | Specification |
|---|---|
| Target | |
| Alternative names | Oxoeicosanoid receptor 1; 5-oxo-ETE G protein-coupled receptor; G protein-coupled receptor 170; G protein-coupled receptor R527; G protein-coupled receptor TG1019; OXER1; GPR170; TG1019 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human OXER1 recombinant protein (Position: M1-Q422). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects oxoeicosanoid receptor 1 (gene OXER1) in human samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 45.8 kDa, and the supplier reports an observed band at about 43–46 kDa.
OXER1 is a G protein-coupled receptor for 5-oxo-ETE and related arachidonic acid metabolites. It signals mainly through Gi/Go proteins and is expressed on eosinophils and other leukocytes, where 5-oxo-ETE acts as a potent chemoattractant, so it is studied in allergic inflammation.
| Target | Oxoeicosanoid receptor 1 (gene OXER1; UniProt Q8TDS5, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 45.8 kDa |
| Observed MW | 43–46 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human THP-1 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): U937 cells.
Immunogen
Recombinant human OXER1 fragment (Met1–Gln422), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places oxoeicosanoid receptor 1 in the membrane. Tissue expression noted by UniProt (human): Expressed in various tissues except brain. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
