Anti-P Glycoprotein/ABCB1 Antibody

SKU:BHA21000021
Research Validated
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Overview
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Anti-ABCB1 antibody (Rabbit polyclonal, Rabbit IgG). Validated for immunohistochemistry. Reactivity: Human, Mouse, Rat (species coverage varies by application). Recognizes ATP-dependent translocase ABCB1. Supplied in lyophilized form, ready for use in standard immunodetection workflows.
Target ABCB1
Host Rabbit
Reactivity Human,Mouse,Rat
Isotype Rabbit IgG
Application(s) IHC
Options selector
Catalog no. Size Conjugation
RP1034 100 ug/vial
Available Options

Select the variant that best fits your experiment.

  • Options:
    • 100 ug/vial / Carrier Free, Unconjugated
      Form: Lyophilized
      Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
      Applications: IHC
      Application details: Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Mouse, Rat, -
      Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat
      Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
    • 100 ug/vial / APC, Biotin, Cy3, FITC, Fluoro488, Fluoro550, Fluoro594, Fluoro647, PE
      Form: Liquid
      Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.; At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
      Applications: WB,IHC,ELISA; Flow Cytometry
      Application details: Western blot, 0.25-0.5μg/ml
      Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml
      ELISA, 0.1-0.5μg/ml
      Flow Cytometry, 1-3μg/1x106 cellsContents: Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
    • 100 ug/vial / HRP
      Form: Liquid
      Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.
      Applications: WB,IHC,ELISA
      Application details: Western blot, 0.25-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml
      ELISA, 0.1-0.5μg/ml
      Contents: Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4.
  • Lead time: typically ships in ~2-3 business days; timing may vary by selected option.
  • Storage: varies by selected option; see option details under Options.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target ABCB1
Alternative names Multidrug resistance protein 1;3.6.3.44;ATP-binding cassette sub-family B member 1;P-glycoprotein 1;CD243;ABCB1;MDR1, PGY1;
UniProt # P08183
Host Rabbit
Clonality
  • Polyclonal
Isotype
  • Rabbit IgG
Reactivity
  • Human
  • Mouse
  • Rat
Applications
  • Immunohistochemistry
Immunogen A synthetic peptide corresponding to a sequence in the middle region of human P Glycoprotein, different from the related rat sequence by twelve amino acids.
Molecular weight 141.5 kDa
Purification Immunogen affinity purified.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Cellular localization Cell membrane ; Multi-pass membrane protein .
Concentration Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form Lyophilized
Storage Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) RP1034
Main SKU BHA21000021
Rabbit Polyclonal

Product Overview

This rabbit polyclonal antibody targets ATP-dependent translocase ABCB1 in human, mouse and rat samples and is validated for IHC. The predicted molecular weight is 141.5 kDa.

P-glycoprotein (ABCB1, MDR1) is an ATP-powered efflux pump in the plasma membrane that expels a wide range of drugs and moves certain phospholipids across the membrane. High ABCB1 levels are a classic cause of multidrug resistance in cancer cells, and it also shapes drug absorption and the blood-brain barrier.

Target ATP-dependent translocase ABCB1 (gene ABCB1; UniProt P08183, human)
Host / Clonality / Isotype Rabbit / Polyclonal / Rabbit IgG
Reactivity Human, Mouse, Rat
Form Lyophilized
Formulation Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg thimerosal; 0.05 mg NaN3; plus stabilizers
Calculated MW 141.5 kDa
Storage As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles.

Validated Applications

IHC (frozen sections) 0.5–1 µg/mL (Mouse, Rat)
IHC (paraffin sections) 0.5–1 µg/mL (Human, Mouse, Rat)

Samples with confirmed staining (IHC): human lung cancer tissue, mouse kidney tissue, rat kidney tissue.

Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.

Samples with confirmed staining (IHC): mouse intestine tissue, rat kidney tissue.

Immunogen

Synthetic peptide from the middle region of human P Glycoprotein. It differs from the rat sequence at twelve residues.

Reactivity Notes

The supplier lists reactivity with human, mouse and rat. UniProt places ATP-dependent translocase ABCB1 in the cell membrane, apical cell membrane and cytoplasm. Tissue expression noted by UniProt (human): Expressed in small intestine.

Safety

Handling: the formulation contains sodium azide and thimerosal (a mercury compound) as preservatives. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: IHC (paraffin sections) 0.5–1 µg/mL (Human, Mouse, Rat); IHC (frozen sections) 0.5–1 µg/mL (Mouse, Rat). Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Human, Mouse, Rat. Other species are not listed by the supplier.
Q.Has this antibody been tested on goat samples?
A.Reactivity with goat samples has not been tested by the supplier. Aligning the immunogen sequence with the goat ortholog (e.g., by BLAST) helps estimate the chance of cross-reactivity.
Q.What immunogen was used to raise this antibody?
A.Synthetic peptide from the middle region of human P Glycoprotein. It differs from the rat sequence at twelve residues.
Q.How should this antibody be stored?
A.As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

Ruizan Shi et al. (2019) Downregulation of cytokeratin 18 enhances BCRP-mediated multidrug resistance through induction of epithelial-mesenchymal transition and predicts poor prognosis in breast cancer. Oncology Reports. 10.3892/or.2019.7069

Jing Huang et al. (2021) Combined effects of low‑dose gambogic acid and NaI131 in drug‑resistant non‑small cell lung cancer cells. Oncology Letters. 10.3892/ol.2021.12849

Xu Wang et al. (2012) Gambogic Acid as a Non-Competitive Inhibitor of ATP-Binding Cassette Transporter B1 Reverses the Multidrug Resistance of Human Epithelial Cancers by Promoting ATP-Binding Cassette Transporter B1 Protein Degradation. Basic & Clinical Pharmacology & Toxicology. 10.1111/j.1742-7843.2012.00921.x

Feng Liang et al. (2017) Overexpression of ILK promotes temozolomide resistance in glioma cells. Molecular Medicine Reports. 10.3892/mmr.2017.6157

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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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