| Field | Specification |
|---|---|
| Target | |
| Alternative names | Multidrug resistance protein 1;3.6.3.44;ATP-binding cassette sub-family B member 1;P-glycoprotein 1;CD243;ABCB1;MDR1, PGY1; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human P Glycoprotein, different from the related rat sequence by twelve amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets ATP-dependent translocase ABCB1 in human, mouse and rat samples and is validated for IHC. The predicted molecular weight is 141.5 kDa.
P-glycoprotein (ABCB1, MDR1) is an ATP-powered efflux pump in the plasma membrane that expels a wide range of drugs and moves certain phospholipids across the membrane. High ABCB1 levels are a classic cause of multidrug resistance in cancer cells, and it also shapes drug absorption and the blood-brain barrier.
| Target | ATP-dependent translocase ABCB1 (gene ABCB1; UniProt P08183, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg thimerosal; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 141.5 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (frozen sections) | 0.5–1 µg/mL (Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
Samples with confirmed staining (IHC): human lung cancer tissue, mouse kidney tissue, rat kidney tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Samples with confirmed staining (IHC): mouse intestine tissue, rat kidney tissue.
Immunogen
Synthetic peptide from the middle region of human P Glycoprotein. It differs from the rat sequence at twelve residues.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places ATP-dependent translocase ABCB1 in the cell membrane, apical cell membrane and cytoplasm. Tissue expression noted by UniProt (human): Expressed in small intestine.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Ruizan Shi et al. (2019) Downregulation of cytokeratin 18 enhances BCRP-mediated multidrug resistance through induction of epithelial-mesenchymal transition and predicts poor prognosis in breast cancer. Oncology Reports. 10.3892/or.2019.7069
Jing Huang et al. (2021) Combined effects of low‑dose gambogic acid and NaI131 in drug‑resistant non‑small cell lung cancer cells. Oncology Letters. 10.3892/ol.2021.12849
Xu Wang et al. (2012) Gambogic Acid as a Non-Competitive Inhibitor of ATP-Binding Cassette Transporter B1 Reverses the Multidrug Resistance of Human Epithelial Cancers by Promoting ATP-Binding Cassette Transporter B1 Protein Degradation. Basic & Clinical Pharmacology & Toxicology. 10.1111/j.1742-7843.2012.00921.x
Feng Liang et al. (2017) Overexpression of ILK promotes temozolomide resistance in glioma cells. Molecular Medicine Reports. 10.3892/mmr.2017.6157