| Field | Specification |
|---|---|
| Target | |
| Alternative names | Multidrug resistance protein 1; ATP-binding cassette sub-family B member 1; P-glycoprotein 1; CD243; ABCB1; MDR1; PGY1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human P Glycoprotein/ABCB1 recombinant protein (Position: R669-Y710). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects ATP-dependent translocase ABCB1 in human, mouse and rat samples and is validated for Western blot, IHC, flow cytometry and ELISA. The predicted molecular weight is 141.5 kDa, and the supplier reports an observed band at about 140 kDa.
P-glycoprotein (ABCB1, MDR1) is an ATP-powered efflux pump in the plasma membrane that expels a wide range of drugs and moves certain phospholipids across the membrane. High ABCB1 levels are a classic cause of multidrug resistance in cancer cells, and it also shapes drug absorption and the blood-brain barrier.
| Target | ATP-dependent translocase ABCB1 (gene ABCB1; UniProt P08183, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 141.5 kDa |
| Observed MW | 140 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): rat PC-12 cells, mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human liver cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed signal (flow cytometry): U2OS cells.
Immunogen
Recombinant human P Glycoprotein/ABCB1 fragment (Arg669–Tyr710), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places ATP-dependent translocase ABCB1 in the cell membrane, apical cell membrane and cytoplasm. Tissue expression noted by UniProt (human): Expressed in small intestine. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Haimeng Pei et al. (2025) Time-Resolved Quantification of Single-Cell Extracellular Vesicles Secretion for Drug Resistance Evaluation. Analytical Chemistry. 10.1021/acs.analchem.5c01558
Xiaoying Wang et al. (2022) Evaluation and antitumor mechanism of functionalized chitosan-based polymeric micelles for oral delivery of paclitaxel. International Journal of Pharmaceutics. 10.1016/j.ijpharm.2022.122138
Jing-Jing Zhang et al. (2010) Design and Implementation of Electrochemical Cytosensor for Evaluation of Cell Surface Carbohydrate and Glycoprotein. Analytical Chemistry. 10.1021/ac9026127
Jinghua Duan et al. (2012) Reversion of multidrug resistance by co-encapsulation of doxorubicin and curcumin in chitosan/poly(butyl cyanoacrylate) nanoparticles. International Journal of Pharmaceutics. 10.1016/j.ijpharm.2012.01.020
Qiuzhi Gao et al. (2024) Gastric cancer mesenchymal stem cells promote tumor glycolysis and chemoresistance by regulating B7H3 in gastric cancer cells. Journal of Cellular Biochemistry. 10.1002/jcb.30521
Bian-Sheng Ji et al. (2013) In vitro and in vivo study of dolichyl phosphate on the efflux activity of P-glycoprotein at the blood–brain barrier. International Journal of Developmental Neuroscience. 10.1016/j.ijdevneu.2013.10.005
Sun Li et al. (2005) Regulation of Multidrug Resistance by MGr1-Antigen in Gastric Cancer Cells. Tumor Biology. 10.1159/000090153
Hong-Bin Xu et al. (2016) Regulation of P-glycoprotein efflux activity by Z-guggulsterone of Commiphora mukul at the blood-brain barrier. Journal of the Neurological Sciences. 10.1016/j.jns.2016.02.046
Cen Juan et al. (2013) Alteration in P-glycoprotein at the blood–brain barrier in the early period of MCAO in rats. Journal of Pharmacy and Pharmacology. 10.1111/jphp.12033
Wu Jing et al. (2010) Glutathione depletion upregulates P-glycoprotein expression at the blood-brain barrier in rats. Journal of Pharmacy and Pharmacology. 10.1211/jpp.61.06.0016
