| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Multidrug resistance protein 1A;3.6.3.44;ATP-binding cassette sub-family B member 1A;MDR1A;Multidrug resistance protein 3;P-glycoprotein 3;Abcb1a;Abcb4, Mdr1a, Mdr3, Pgy-3, Pgy3; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | Mixture of human and hamster drug-resistant whole cells and crude plasma membranes. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-P-GlycoProtein(MDR) ABCB1 Antibody (Monoclonal, F4) is an antibody targeting Abcb1a. Common applications include WB, IHC, ICC. Key specifications include host: Mouse; clonality: Monoclonal; clone: Clone: F4; isotype: Mouse IgG1; reactivity: Human; observed MW: 200 kDa; calculated MW: 140647 MW.
Boster Bio Anti-P-GlycoProtein (MDR) ABCB1 Antibody (Monoclonal, F4) catalog # MA1060. Tested in IHC, ICC, WB applications. This antibody reacts with Human.
Key elements and design rationale
- Target: Abcb1a — DNA helicase
- Antibody format: Host: Mouse; Clonality: Monoclonal; Clone: Clone: F4; Isotype: Mouse IgG1
- Species reactivity: Human
- Molecular weight guidance: Observed: 200 kDa; Calculated: 140647 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells. .
Scientific background (datasheet): P-Glycoprotein,, also known as Multidrug Resistance 1 (MDR1), is one of the ATP-binding cassette transporters family. P-glycoprotein-1 is involved in the transport of 3 of these protease inhibitors in vitro. MDR1 gene is mapped to the 7q21.1 by in situ hybridization. The MDR1 gene product, P-glycoprotein, mediates the transport of the cardiac glycoside, digoxin.
Cellular localization (datasheet): Cell membrane; Multi-pass membrane protein.
Tissue details (datasheet): ABCB1: Expressed in liver, kidney, small intestineand brain.
Sequence similarities (datasheet): Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
Research relevance and current trends
- Commonly studied in contexts related to ATPases,Cancer,Lipid Metabolism,Metabolism,Nuclear Import/Export,Plasma Membrane,Protein Trafficking,Signal Transduction.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
- Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
