| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Protein NDRG3;N-myc downstream-regulated gene 3 protein;NDRG3; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminusof human P2Y6/P2RY6, which shares 94.4% amino acid (aa) sequence identity with rat P2RY6. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-P2Y6/P2RY6 Antibody Picoband® is an antibody for P2RY6 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: P2RY6 (Protein NDRG3); UniProt: Q15077
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 45 kDa, calculated 41409 MW
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-P2Y6/P2RY6 Antibody Picoband® catalog # A10087-2.
Biological background
Biological context: Promotes the growth of epithelial cells. May stimulate the phosphorylation of EGFR and mitogen-activated protein kinases.
Expression and localization notes: cellular localization: Membrane ; Single-pass type I membrane protein ., tissue context: Ubiquitous. Highly expressed in brain. ..
Common research applications
- Western blotting (WB): Compare P2RY6 levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of P2RY6 in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify P2RY6-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: P2Y purinoceptor 6 is a protein that in humans is encoded by the P2RY6 gene. The product of this gene belongs to the family of P2 receptors, which is activated by extracellular nucleotides and subdivided into P2X ligand-gated ion channels and P2Y G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor, which is a G-protein coupled receptor, is responsive to UDP, partially responsive to UTP and ADP, and not responsive to ATP. It is proposed that this receptor mediates inflammatory responses. Alternative splicing results in multiple transcript variants that encode different protein isoforms.
- Cross reactivity: No cross-reactivity with other proteins
- Cellular localization: Membrane ; Single-pass type I membrane protein .
- Tissue details: Ubiquitous. Highly expressed in brain. .
- Research category: Cancer,Cell Biology,Cell Cycle,Cell Differentiation,Tumor Biomarkers
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
