Anti-p38 MAPK (Phospho-Tyr182) Antibody

SKU:BHA10918548
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    Overview
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    Clonality Polyclonal
    Species Reactivity Human,Mouse,Rat
    Validated Applications Western Blot, Immunohistochemistry
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    Catalog no. Size
    Y011253 100 ug
    Field Specification
    Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using; epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed; by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

    Product Overview

    Research Use Only

    p38 MAPK (phospho-Tyr182) antibody detects endogenous levels of P38MAPK only when phosphorylated at tyrosine 182.

    Validated Applications

    Western Blot (WB) 1:500~1:1000 IHC: 1:50-1:100
    Immunohistochemistry (IHC) 1:50-1:100

    Key Specifications

    Clonality Polyclonal
    Species Reactivity Human,Mouse,Rat
    Concentration 1ug/ul
    Formulation Liquid
    Catalog No. Y011253

    Immunogen / Antigen

    The antiserum was produced against synthesized phosphopeptide derived from human p38 MAPK around the phosphorylation site of tyrosine 182 (T-G-YP-V-A).

    Reactivity Notes

    p38 MAPK (phospho-Tyr182) antibody detects endogenous levels of P38MAPK only when phosphorylated at tyrosine 182.

    Additional Information

    Application Notes: WB: 1:500~1:1000 IHC: 1:50-1:100

    Storage & Handling

    This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

    Safety

    For Research Use Only (RUO). Not intended for diagnostic, therapeutic, or veterinary use. Handle according to standard laboratory biosafety guidelines. Avoid skin and eye contact. Dispose of waste in accordance with local regulations.

    What applications has the Anti-p38 MAPK (Phospho-Tyr182) Antibody been validated for?

    This antibody has been validated for: Western Blot, Immunohistochemistry. For each application, follow the recommended dilutions on the product datasheet and optimize empirically for your sample type.

    What is the recommended dilution range?

    WB: 1:500~1:1000 IHC: 1:50-1:100

    Which species does this antibody recognize?

    This antibody has been confirmed reactive against: Human,Mouse,Rat. Cross-reactivity with other species may occur due to sequence homology but is not guaranteed without independent validation.

    What is the immunogen / antigen used to generate this antibody?

    The antiserum was produced against synthesized phosphopeptide derived from human p38 MAPK around the phosphorylation site of tyrosine 182 (T-G-YP-V-A).

    How should this antibody be stored and handled?

    This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt. Avoid repeated freeze-thaw cycles. Centrifuge vials briefly before opening. Dilute immediately before use.

    Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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