Anti-P53 Tp53 Monoclonal Antibody

Overview

Anti-P53 Tp53 Monoclonal Antibody is an antibody targeting TP53. Common applications include WB, IHC, ICC. Key specifications include host: Mouse; clonality: Monoclonal; clone: Clone: BP53-12; isotype: Mouse IgG2a; reactivity: Human; observed MW: 53 kDa; calculated MW: 43451 MW.

Boster Bio Anti-P53 Tp53 Monoclonal Antibody catalog # MA1078. Tested in IHC, ICC, WB applications. This antibody reacts with Human.

Key elements and design rationale

• Target: TP53 — Cellular tumor antigen p53
• Antibody format: Host: Mouse; Clonality: Monoclonal; Clone: Clone: BP53-12; Isotype: Mouse IgG2a
• Species reactivity: Human
• Molecular weight guidance: Observed: 53 kDa; Calculated: 43451 MW

Specificity note: No cross reactivity with other proteins.

Biological background

Protein function (datasheet): Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA- Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2. .

Scientific background (datasheet): The p53 tumor antigen is found in increased amounts in a wide variety of transformed cells. The protein is also detectable in many actively proliferating, nontransformed cells, but it is undetectable or present at low levels in resting cells. This protein induces cell cycle arrest or apoptosis in response to sublethal or severe DNA damage, respectively, by differential transcription of target genes and through transcription-independent apoptotic functions. The p53 protein contains 393 amino acids. Human p53 tumour antigen is Locatedto band 17p13. p53 mutations are common in pancreatic cancer and are absent in chronic pancreatitis.

Cellular localization (datasheet): Cytoplasm . Nucleus . Endoplasmic reticulum . Mitochondrion matrix . Interaction with BANP promotes nuclear localization. Translocates to mitochondria upon oxidative stress (By similarity). .

Tissue details (datasheet): TP53: Ubiquitous. Isoforms are expressed in a widerange of normal tissues but in a tissue-dependent manner. Isoform2 is expressed in most normal tissues but is not detected inbrain, lung, prostate, muscle, fetal brain, spinal cord and fetalliver. Isoform 3 is expressed in most normal tissues but is notdetected in lung, spleen, testis, fetal brain, spinal cord andfetal liver. Isoform 7 is expressed in most normal tissues but isnot detected in prostate, uterus, skeletal muscle and breast.Isoform 8 is detected only in colon, bone marrow, testis, fetalbrain and intestine. Isoform 9 is expressed in most normal tissuesbut is not detected in brain, heart, lung, fetal liver, salivarygland, breast or intestine.

Sequence similarities (datasheet): Belongs to the p53 family.

Research relevance and current trends

• Commonly studied in contexts related to Apoptosis,Cell Biology,Intracellular,p53 Pathway.
• Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
• Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).

Common research applications

• Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
• Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
• Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.

Notes for experimental interpretation

• Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
• Cross-reactivity (datasheet): No cross-reactivity with other proteins
• Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.

As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.