| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Protein CIP2A;Cancerous inhibitor of PP2A;p90 autoantigen;KIAA1524;CIP2A; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human KIAA1524, different from the related mouse sequence by three amino acids. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of CIP2A (Protein CIP2A) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-p90 Autoantigen/KIAA1524 CIP2A Antibody Picoband® catalog # PB9714. Tested in WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the C-terminus of human KIAA1524, different from the related mouse sequence by three amino acids.
- Molecular weight context: reported MW: 90 kDa; calculated MW: 102185 MW
- Reactivity: Human,Mouse
- Applications: WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Protein CIP2A; Protein CIP2A. Protein CIP2A, also known as cancerous inhibitor of PP2A (CIP2A), is a protein that in humans is encoded by the KIAA1524 gene. CIP2A is required for the malignant cellular growth and for in vivo tumor formation. In accordance with the oncogenic role of CIP2A, overexpression of CIP2A promotes Ras-elicited cell growth and transforms immortalized human cells (HEK-TERVs). More recently CIP2A has been shown to regulate phosphorylation and activity of many other oncoproteins and to drive malignant cell growth and tumorigenesis in various human cancer types. Importantly, CIP2A deficient mice are viable, suggesting that targeting of oncogenic function of CIP2A would not result in serious side-effects. Functional note: Oncoprotein that inhibits PP2A and stabilizes MYC in human malignancies. Promotes anchorage-independent cell growth and tumor formation. . Reported localization: Membrane ; Single-pass membrane protein . Cytoplasm . Slightly concentrates in the perinuclear region. Expression/tissue context: Expressed at low levels in most of the tissues. Overexpressed in head-and-neck squamous cell carcinomas (HNSCC). Present in liver cancer cells (at protein level). .
Research relevance and current trends
- Cancer: Researchers commonly examine how CIP2A (Protein CIP2A) relates to this theme using model systems and orthogonal readouts.
- Oncoproteins: Researchers commonly examine how CIP2A (Protein CIP2A) relates to this theme using model systems and orthogonal readouts.
- Oncoproteins/Suppressors: Researchers commonly examine how CIP2A (Protein CIP2A) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative CIP2A (Protein CIP2A) levels across conditions; band patterns may reflect isoforms and processing.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
