| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Keratin, type II cytoskeletal 8;Cytokeratin endo A;Cytokeratin-8;CK-8;Keratin-8;K8;Type-II keratin Kb8;Krt8;Krt2-8; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | Cytokeratin from human epidermis. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Pan cytokeratin Antibody (Monoclonal, PCK-26) is an antibody targeting Krt8. Common applications include WB, IHC, IF. Key specifications include host: Mouse; clonality: Monoclonal; clone: Clone: PCK-26; isotype: Mouse IgG1; reactivity: Human,Mouse; observed MW: 54 kDa; calculated MW: 54019 MW.
Boster Bio Anti-Pan cytokeratin Antibody (Monoclonal, PCK-26) catalog # MA1082. Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse.
Key elements and design rationale
- Target: Krt8 — Keratin, type II cytoskeletal 8
- Antibody format: Host: Mouse; Clonality: Monoclonal; Clone: Clone: PCK-26; Isotype: Mouse IgG1
- Species reactivity: Human,Mouse
- Molecular weight guidance: Observed: 54 kDa; Calculated: 54019 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. .
Scientific background (datasheet): Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues.
Cellular localization (datasheet): Cytoplasm . Nucleus, nucleoplasm . Nucleus matrix .
Tissue details (datasheet): Expressed in cardiac and striated muscle. Expressed at Z-lines within the muscle fibers and at Z-line and M- line domains at costameres at the sarcolemmal membrane (at protein level). Observed in coagulating gland, bladder, salivary gland, kidney, spleen, thymus, lung and heart. Also observed in ventral prostate, seminal vesicle and liver where expression increases following castration. .
Sequence similarities (datasheet): Belongs to the intermediate filament family.
Research relevance and current trends
- Commonly studied in contexts related to Class I,Cytoskeleton,Cytoskeleton/ECM,Intermediate Filaments,Keratin,Signal Transduction.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
- Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
