| Field | Specification |
|---|---|
| Target | |
| Alternative names | PAN2-PAN3 deadenylation complex catalytic subunit PAN2; Inactive ubiquitin carboxyl-terminal hydrolase 52; PAB1P-dependent poly(A)-specific ribonuclease; Poly(A)-nuclease deadenylation complex subunit 2; PAN deadenylation complex subunit 2; PAN2; KIAA0710; USP52 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human PAN2 recombinant protein (Position: M1-L356). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects PAN2-PAN3 deadenylation complex catalytic subunit PAN2 in human, mouse and rat samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 135.4 kDa, and the supplier reports an observed band at about 135 kDa.
PAN2 is the catalytic subunit of the PAN2–PAN3 deadenylase, a cytoplasmic enzyme that trims mRNA poly(A) tails. It acts early in mRNA decay before the CCR4–NOT complex and is stimulated by poly(A)-binding protein.
| Target | PAN2-PAN3 deadenylation complex catalytic subunit PAN2 (gene PAN2; UniProt Q504Q3, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 135.4 kDa |
| Observed MW | 135 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human, Mouse, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1x1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HepG2 cells, human Hel cells, human Daudi cells, rat PC-12 cells, mouse thymus tissue, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.25 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): PC-3 cells.
Immunogen
Recombinant human PAN2 fragment (Met1–Leu356), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places PAN2-PAN3 deadenylation complex catalytic subunit PAN2 in the cytoplasm and nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
