| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | CD59 glycoprotein;1F5 antigen;20 kDa homologous restriction factor;HRF-20;HRF20;MAC-inhibitory protein;MAC-IP;MEM43 antigen;Membrane attack complex inhibition factor;MACIF;Membrane inhibitor of reactive lysis;MIRL;Protectin;CD59;CD59;MIC11, MIN1, MIN2, MIN3, MSK21; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human Pannexin 1 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-PANX1 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 24P11; isotype IgG; reactivity: Human. Reported application contexts include WB, IP (as provided in the source record). Boster Bio Anti-Pannexin 1 Rabbit Monoclonal Antibody catalog # M00915. Tested in WB, IP applications. This antibody reacts with Human.
Key elements and design rationale
- Target: PANX1 (CD59 glycoprotein).
- Antibody format: Monoclonal; clone 24P11; isotype IgG.
- Host: Rabbit.
- Species reactivity: Human (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
PANX1 (protein: Glycogen synthase kinase-3 beta (gsk3b)) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase. Reported cellular localization context: Cell membrane; Lipid-anchor, GPI-anchor. Secreted. Soluble form found in a number of tissues. Tissue expression notes (as provided): Detected in caudate, nucleus accumbens and in the olfactory tubercle. .
Research relevance and current trends
- Research context keywords from the source record include: Atherosclerosis,Cardiovascular,Cell Type Markers,Complement,Hematopoietic Progenitors,Immunology,Innate Immunity,Protein Phosphorylation,Signal Transduction,Stem Cells,Surface Molecules,Tyrosine Kinases,Vascular Inflammation.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.
Workflow ideas (metafield): Validate PANX1 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect PANX1 expression by Western blot in cell or tissue lysates, Enrich PANX1 by immunoprecipitation from lysates for downstream analysis
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 48 kDa; calculated MW: 14177 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 48 kDa
- Cellular localization (provided): Cell membrane; Lipid-anchor, GPI-anchor. Secreted. Soluble form found in a number of tissues.
- Tissue details (provided): Detected in caudate, nucleus accumbens and in the olfactory tubercle. .
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
