| Field | Specification |
|---|---|
| Target | |
| Alternative names | Proteinase-activated receptor 2; PAR-2; Coagulation factor II receptor-like 1; G protein-coupled receptor 11; Thrombin receptor-like 1; F2RL1; GPR11; PAR2 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human PAR2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 22F95 is a rabbit monoclonal antibody that recognizes proteinase-activated receptor 2 (gene F2RL1) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 44.1 kDa, and the supplier reports an observed band at about 50 kDa.
Proteinase-activated receptor 2 (PAR2, encoded by F2RL1) is a G protein-coupled receptor activated by trypsin, tryptase and other trypsin-like proteases, which cleave it to unmask a tethered ligand. PAR2 signals through calcium, MAPK and NF-κB pathways and is studied in inflammation, pain, skin and gut biology.
| Target | Proteinase-activated receptor 2 (gene F2RL1; UniProt P55085, human) |
|---|---|
| Clone | 22F95 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 44.1 kDa |
| Observed MW | 50 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): human PC-3 cells, human A549 cells, human K562 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human colon adenocarcinoma tissue, human liver cancer tissue, human placenta tissue, human spleen tissue, human thyroid papillary carcinoma tissue, human urothelial carcinoma tissue, non-small cells lung cancer tissue, rat small intestine tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human PAR2.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places proteinase-activated receptor 2 in the cell membrane. Tissue expression noted by UniProt (human): Widely expressed in tissues with especially high levels in pancreas, liver, kidney, small intestine, and colon. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Jing Lu et al. (2014) Tryptase inhibitor APC 366 prevents hepatic fibrosis by inhibiting collagen synthesis induced by tryptase/protease-activated receptor 2 interactions in hepatic stellate cells. International Immunopharmacology. 10.1016/j.intimp.2014.04.001
