| Field | Specification |
|---|---|
| Target | |
| Alternative names | Protein deglycase DJ-1 ;DJ-1;3.1.2.- ;3.5.1.- ;Oncogene DJ1;Parkinson disease protein 7;PARK7 ; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human PARK7 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone GAE-16 is a rabbit monoclonal antibody that recognizes parkinson disease protein 7 (gene PARK7) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 19.9 kDa, and the supplier reports an observed band at about 20 kDa.
DJ-1 (PARK7) is a small, widely expressed protein that protects cells against oxidative stress. It senses oxidation through a key cysteine and supports antioxidant responses, including stabilization of NRF2; loss-of-function mutations cause a rare early-onset form of Parkinson's disease.
| Target | Parkinson disease protein 7 (gene PARK7; UniProt Q99497, human) |
|---|---|
| Clone | GAE-16 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 19.9 kDa |
| Observed MW | 20 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): human HeLa cells, human HEL cells, human U251 cells, human HepG2 cells, rat brain tissue, rat C6 cells, mouse brain tissue, mouse Neuro-2a cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:1000 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Samples with confirmed staining (IHC): human bladder cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human PARK7.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places parkinson disease protein 7 in the cell membrane, cytoplasm, nucleus and membrane raft. Tissue expression noted by UniProt (human): Highly expressed in pancreas, kidney, skeletal muscle, liver, testis and heart. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Wei Ni et al. (2022) Neural stem cell secretome exerts a protective effect on damaged neuron mitochondria in Parkinson's disease model. Brain Research. 10.1016/j.brainres.2022.147978
