| Field | Specification |
|---|---|
| Target | |
| Alternative names | Protein deglycase DJ-1 ;DJ-1;3.1.2.- ;3.5.1.- ;Oncogene DJ1;Parkinson disease protein 7;PARK7 ; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human PARK7 recombinant protein (Position: A2-D189). Human PARK7 shares 91% amino acid (aa) sequence identity with both mouse and rat PARK7. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets parkinson disease protein 7 (gene PARK7) in human, mouse and rat samples and is validated for immunoprecipitation, IHC, ICC/IF and Western blot. The predicted molecular weight is 19.9 kDa, and the supplier reports an observed band at about 20 kDa.
DJ-1 (PARK7) is a small, widely expressed protein that protects cells against oxidative stress. It senses oxidation through a key cysteine and supports antioxidant responses, including stabilization of NRF2; loss-of-function mutations cause a rare early-onset form of Parkinson's disease.
| Target | Parkinson disease protein 7 (gene PARK7; UniProt Q99497, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3; plus stabilizers |
| Calculated MW | 19.9 kDa |
| Observed MW | 20 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Immunoprecipitation | 0.5–2 µg/mL (Human) |
Samples with a confirmed band (WB): human 293T cells, human PANC-1 cells, human HeLa cells, human U251 cells, rat kidney tissue, mouse kidney tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): SK-OV-3 cells.
Samples with a confirmed result (IP): HeLa cells.
Immunogen
Recombinant human PARK7 fragment (Ala2–Asp189), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 91%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places parkinson disease protein 7 in the cell membrane, cytoplasm, nucleus and membrane raft. Tissue expression noted by UniProt (human): Highly expressed in pancreas, kidney, skeletal muscle, liver, testis and heart. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
