| Field | Specification |
|---|---|
| Target | |
| Alternative names | Protein DJ-1; DJ-1; Contraception-associated protein 1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived rat PARK7 / DJ1 recombinant protein (Position: A2-D189). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets parkinson disease protein 7 homolog (gene Park7) in mouse and rat samples and is validated for ELISA, IHC and Western blot. The predicted molecular weight is 20.0 kDa, and the supplier reports an observed band at about 20 kDa.
DJ-1 (PARK7) is a small, widely expressed protein that protects cells against oxidative stress. It senses oxidation through a key cysteine and supports antioxidant responses, including stabilization of NRF2; loss-of-function mutations cause a rare early-onset form of Parkinson's disease.
| Target | Parkinson disease protein 7 homolog (gene Park7; UniProt O88767, rat) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 20.0 kDa |
| Observed MW | 20 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL |
| ELISA (capture antibody) | 1–5 µg/mL |
Samples with a confirmed band (WB): rat liver tissue, rat RH35 cells, mouse kidney tissue, mouse liver tissue, mouse Hepa1/6 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse liver tissue, mouse pancreas tissue, mouse testis tissue, rat liver tissue, rat pancreas tissue, rat testis tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Immunogen
Recombinant rat PARK7 / DJ1 fragment (Ala2–Asp189), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places parkinson disease protein 7 homolog in the cell membrane, cytoplasm, membrane raft and nucleus. Tissue expression noted by UniProt (rat): Ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yumei Li et al. (2023) DJ-1 inhibits ferroptosis in cerebral ischemia-reperfusion via ATF4/HSPA5 pathway. Neurochemistry International. 10.1016/j.neuint.2023.105628
