| Field | Specification |
|---|---|
| Target | |
| Alternative names | Poly [ADP-ribose] polymerase 2; PARP-2; hPARP-2; ADP-ribosyltransferase diphtheria toxin-like 2; ARTD2; DNA ADP-ribosyltransferase PARP2; NAD(+) ADP-ribosyltransferase 2; ADPRT-2; Poly[ADP-ribose] synthase 2; pADPRT-2; Protein poly-ADP-ribosyltransferase PARP2; PARP2; ADPRT2; ADPRTL2 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human PARP2 recombinant protein (Position: R13-E253). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes poly (gene PARP2) in human and rat samples and is validated for Western blot, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 66.2 kDa, and the supplier reports an observed band at about 66 kDa.
PARP2 is a nuclear poly(ADP-ribose) polymerase closely related to PARP1. It is activated by DNA breaks and adds ADP-ribose chains to proteins to support DNA repair, and together with PARP1 it is inhibited by clinical PARP inhibitors.
| Target | Poly (gene PARP2; UniProt Q9UGN5, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3 |
| Calculated MW | 66.2 kDa |
| Observed MW | 66 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Rat) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): rat testis tissue, human HEK293 cells, human HL-60 cells, human U2OS cells, human HepG2 cells, human A431 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): HepG2 cells.
Samples with a confirmed signal (flow cytometry): HL-60 cells.
Immunogen
Recombinant human PARP2 fragment (Arg13–Glu253), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places poly in the nucleus and chromosome. Tissue expression noted by UniProt (human): Widely expressed, mainly in actively dividing tissues. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
