| Field | Specification |
|---|---|
| Target | |
| Alternative names | Alpha-parvin;Actopaxin;CH-ILKBP;Calponin-like integrin-linked kinase-binding protein;Matrix-remodeling-associated protein 2;PARVA;MXRA2; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human Parvin alpha, identical to the related mouse sequence, and different from the related rat sequence by one amino acid. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets alpha-parvin (gene PARVA) in human, mouse and rat samples and is validated for IHC and Western blot. The predicted molecular weight is 42.2 kDa, and the supplier reports an observed band at about 42 kDa.
α-Parvin (PARVA, also called actopaxin) is an actin-binding protein of focal adhesions. As part of the IPP complex with ILK and PINCH, it links integrins to the actin cytoskeleton and supports cell spreading, migration and blood vessel development.
| Target | Alpha-parvin (gene PARVA; UniProt Q9NVD7, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 42.2 kDa |
| Observed MW | 42 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Human) |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
Samples with a confirmed band (WB): rat liver tissue, HeLa cells, SW620 cells, HEPA cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human mammary cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide from the middle region of human parvin alpha. The peptide sequence is conserved in mouse. It differs from the rat sequence at one residue.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places alpha-parvin in the cell junction, cell membrane and cytoplasm. Tissue expression noted by UniProt (human): Widely expressed, with highest levels in heart, skeletal muscle, kidney and liver. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
