| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Paired box protein Pax-6;Aniridia type II protein;Oculorhombin;PAX6;AN2; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthesized peptide derived from human PAX6 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-PAX6 Rabbit Monoclonal Antibody is an antibody targeting PAX6. Common applications include WB, IHC, Flow Cytometry. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: HEB-16; isotype: Rabbit IgG; reactivity: Human,Mouse,Rat; observed MW: 75 kDa; calculated MW: 46683 MW.
Boster Bio Anti-PAX6 Rabbit Monoclonal Antibody catalog # M00273-2. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: PAX6 — Paired box protein Pax-6
- Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: HEB-16; Isotype: Rabbit IgG
- Species reactivity: Human,Mouse,Rat
- Molecular weight guidance: Observed: 75 kDa; Calculated: 46683 MW
Biological background
Protein function (datasheet): Transcription factor with important functions in the development of the eye, nose, central nervous system and pancreas. Required for the differentiation of pancreatic islet alpha cells (By similarity). Competes with PAX4 in binding to a common element in the glucagon, insulin and somatostatin promoters. Regulates specification of the ventral neuron subtypes by establishing the correct progenitor domains (By similarity). Isoform 5a appears to function as a molecular switch that specifies target genes. .
Cellular localization (datasheet): Nucleus.
Tissue details (datasheet): Fetal eye, brain, spinal cord and olfactory epithelium. Isoform 5a is less abundant than the PAX6 shorter form.
Research relevance and current trends
- Commonly studied in contexts related to Cell Type Marker,Developmental Biology,Developmental Families,Domain Families,Epigenetics and Nuclear Signaling,Lineage Markers,Lineage Specification,Neural Signal Transduction,Neurology Process,Neuroscience,Organogenesis,Stem Cells,Transcription.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
- Flow cytometry: Quantify target-positive populations in single-cell suspensions; pair with viability and isotype/FMO controls conceptually.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
