| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Paxillin;Pxn; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Gene ID | |
| Host | |
| Immunogen | C-terminal part of recombinant chicken paxillin (amino acids 305-559). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-Paxillin Pxn Antibody (Monoclonal, PXC-10) is an antibody targeting PXN. Common applications include WB, ICC. Key specifications include host: Mouse; clonality: Monoclonal; clone: Clone: PXC-10; isotype: Mouse IgG1; reactivity: Bovine,Chicken,Hamster,Human,Mouse,Rat; observed MW: 65 kDa; calculated MW: 64019 MW.
Boster Bio Anti-Paxillin Pxn Antibody (Monoclonal, PXC-10) catalog # MA1080. Tested in ICC, WB applications. This antibody reacts with Chicken, Human, Mouse, Rat.
Key elements and design rationale
- Target: PXN — Paxillin
- Antibody format: Host: Mouse; Clonality: Monoclonal; Clone: Clone: PXC-10; Isotype: Mouse IgG1
- Species reactivity: Bovine,Chicken,Hamster,Human,Mouse,Rat
- Molecular weight guidance: Observed: 65 kDa; Calculated: 64019 MW
Specificity note: No cross reactivity with other proteins.
Biological background
Protein function (datasheet): Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). .
Scientific background (datasheet): The paxillin gene can be alternatively spliced to include 1 of 2 alternative exons, generating beta and gamma isoforms. Paxillin is a 68-kDa focal adhesion protein that is phosphorylated on tyrosine residues in fibroblasts in response to transformation by v-src, treatment with platelet-derived growth factor, or cross-linking of integrins. The 68-kD protein (paxillin) is a cytoskeletal component that localizes to the focal adhesions at the ends of actin stress fibers in chicken embryo fibroblasts. It is also present in the focal adhesions of Madin-Darby bovine kidney (MDBK) epithelial cells but is absent, like talin, from the cell-cell adherens junctions of these cells.
Cellular localization (datasheet): Cytoplasm, cytoskeleton . Cell junction, focal adhesion . Cytoplasm, cell cortex . Colocalizes with integrins at the cell periphery. Colocalizes with PXN to membrane ruffles and the leading edge of migrating cells (By similarity). .
Tissue details (datasheet): CDH1: Non-neural epithelial tissues.
Sequence similarities (datasheet): Belongs to the paxillin family.
Research relevance and current trends
- Commonly studied in contexts related to Adapters,Cancer,Cytoplasmic,Cytoskeleton/ECM,Extracellular Matrix,Signal Transduction,Structures.
- Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
- Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).
Common research applications
- Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
- Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
Notes for experimental interpretation
- Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
- Cross-reactivity (datasheet): No cross-reactivity with other proteins
- Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.
As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
