| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Activated RNA polymerase II transcriptional coactivator p15;Positive cofactor 4;PC4;SUB1 homolog;p14;SUB1;PC4, RPO2TC1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human PC4, different from the related mouse and rat sequences by one amino acid. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of SUB1 (Activated RNA polymerase II transcriptional coactivator p15) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-PC4/SUB1 Antibody Picoband® catalog # PB10098. Tested in IF, IHC WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the C-terminus of human PC4, different from the related mouse and rat sequences by one amino acid.
- Molecular weight context: reported MW: 19 kDa; calculated MW: 14395 MW
- Reactivity: Human,Mouse,Rat
- Applications: IF, IHC WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Activated RNA polymerase II transcriptional coactivator p15; Activated RNA polymerase II transcriptional coactivator p15. Activated RNA polymerase II transcriptional coactivator p15, also known as positive cofactor 4 (PC4) or SUB1 homolog, is a protein that in humans is encoded by the SUB1 gene. This gene is mapped to 5p13.3. The transcriptional cofactor PC4 is an ancient single-strand DNA (ssDNA)-binding protein that has a homologue in bacteriophage T5 where it is likely the elusive replicative ssDNA-binding protein. The recombinant PC4 is shown to function identically to the native protein through its interaction with TAFs. Functional note: General coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. May be involved in stabilizing the multiprotein transcription complex. Binds single-stranded DNA. Also binds, in vitro, non-specifically to double-stranded DNA (ds DNA). . Reported localization: Nucleus. Expression/tissue context: Ubiquitous. Expressed in leukocytes and plasma. .
Research relevance and current trends
- Epigenetics and Nuclear Signaling: Researchers commonly examine how SUB1 (Activated RNA polymerase II transcriptional coactivator p15) relates to this theme using model systems and orthogonal readouts.
- Pol II Transcription: Researchers commonly examine how SUB1 (Activated RNA polymerase II transcriptional coactivator p15) relates to this theme using model systems and orthogonal readouts.
- Polymerase Associated Factors: Researchers commonly examine how SUB1 (Activated RNA polymerase II transcriptional coactivator p15) relates to this theme using model systems and orthogonal readouts.
Common research applications
- IF/ICC: evaluate subcellular localization and co-localization patterns; signal can depend on fixation/permeabilization and epitope accessibility.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
