| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Cadherin-5;Vascular endothelial cadherin;VE-cadherin;CD144;Cdh5; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human PCBP1 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-PCBP1 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 25P90; isotype IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, IP (as provided in the source record). Boster Bio Anti-PCBP1 Rabbit Monoclonal Antibody catalog # M02636-1. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: PCBP1 (Cadherin-5).
- Antibody format: Monoclonal; clone 25P90; isotype IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
PCBP1 (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. Acts in concert with KRIT1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction. . Reported cellular localization context: Cell junction . Cell membrane ; Single-pass type I membrane protein . Found at cell-cell boundaries and probably at cell-matrix boundaries. KRIT1 and CDH5 reciprocally regulate their localization to endothelial cell-cell junctions (By similarity). . Tissue expression notes (as provided): Keratinocytes of epidermis and other stratified squamous epithelia.
Research relevance and current trends
- Research context keywords from the source record include: Atherosclerosis,Cadherins,Cancer,Cardiovascular,Cell Adhesion,Cell Adhesion Molecules,Cytoskeleton/ECM,Invasion/Microenvironment,Signal Transduction,Vascular Inflammation,Vasculature.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.
Workflow ideas (metafield): Validate PCBP1 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect PCBP1 expression by Western blot in cell or tissue lysates, Enrich PCBP1 by immunoprecipitation from lysates for downstream analysis
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 40 kDa; calculated MW: 87903 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 40 kDa
- Cellular localization (provided): Cell junction . Cell membrane ; Single-pass type I membrane protein . Found at cell-cell boundaries and probably at cell-matrix boundaries. KRIT1 and CDH5 reciprocally regulate their localization to endothelial cell-cell junctions (By similarity). .
- Tissue details (provided): Keratinocytes of epidermis and other stratified squamous epithelia.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
