| Field | Specification |
|---|---|
| Target | |
| Alternative names | Proprotein convertase subtilisin/kexin type 9; Neural apoptosis-regulated convertase 1; NARC-1; Proprotein convertase 9; PC9; Subtilisin/kexin-like protease PC9; PCSK9; NARC1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human PCSK9 recombinant protein (Position: S153-Q344). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects proprotein convertase subtilisin/kexin type 9 (gene PCSK9) in human samples and is validated for Western blot, IHC and ELISA. The predicted molecular weight is 74.3 kDa; the supplier reports observed bands at 66 kDa, 80 kDa (precursor).
PCSK9 is a secreted proprotein convertase made mainly in the liver that binds the LDL receptor and sends it for lysosomal degradation. By lowering LDL receptor levels it raises plasma LDL cholesterol, which made PCSK9 a major target of cholesterol-lowering antibodies and siRNA drugs.
| Target | Proprotein convertase subtilisin/kexin type 9 (gene PCSK9; UniProt Q8NBP7, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 74.3 kDa |
| Observed MW | 66 kDa, 80 kDa (precursor) |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human) |
| ELISA (capture antibody) | 1–5 µg/mL (Human) |
Samples with a confirmed band (WB): human A431 cells, human HeLa cells, human Caco-2 cells, human HepG2 cells, human Jurkat cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human liver cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human PCSK9 fragment (Ser153–Gln344), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes proprotein convertase subtilisin/kexin type 9 as secreted and also located in the cytoplasm, endosome and lysosome. Tissue expression noted by UniProt (human): Expressed in neuro-epithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
