| Field | Specification |
|---|---|
| Target | |
| Alternative names | Programmed cell death 1 ligand 1;PD-L1;PDCD1 ligand 1;Programmed death ligand 1;B7 homolog 1;B7-H1;CD274;CD274;B7H1, PDCD1L1, PDCD1LG1, PDL1; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human PD-L1 recombinant protein (Position: E45-T290). Human PD-L1 shares 69% amino acid (aa) sequence identity with mouse PD-L1. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets programmed cell death 1 ligand 1 (gene CD274) in human samples and is validated for flow cytometry and Western blot. The predicted molecular weight is 33.3 kDa, and the supplier reports an observed band at about 40–50 kDa.
PD-L1 (CD274) is a transmembrane ligand that binds the inhibitory receptor PD-1 on T cells and limits their activation, helping maintain tolerance to self. Many tumors upregulate PD-L1 to escape immune attack, which made the PD-1/PD-L1 axis a cornerstone of immune checkpoint research.
| Target | Programmed cell death 1 ligand 1 (gene CD274; UniProt Q9NZQ7, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 33.3 kDa |
| Observed MW | 40–50 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human PC-3 cells, human placenta tissue, human HepG2 cells, human THP-1 cells, human K562 cells, human A549 cells, human HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): Jurkat cells.
Immunogen
Recombinant human PD-L1 fragment (Glu45–Thr290), expressed in E. coli. Sequence identity with the mouse ortholog: 69%.
Reactivity Notes
The supplier lists reactivity with human. UniProt places programmed cell death 1 ligand 1 in the cell membrane, early endosome membrane, recycling endosome membrane and nucleus. Tissue expression noted by UniProt (human): Highly expressed in the heart, skeletal muscle, placenta and lung. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Y Zuo et al. (2020) MiR-34a-5p/PD-L1 axis regulates cisplatin chemoresistance of ovarian cancer cells. Neoplasma. 10.4149/neo_2019_190202N106
